Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue

P. A. Usher; A. M. Sieuwerts; A. Bartels; U. Lademann; Hans Jørgen Nielsen; L. Holten-Andersen; J. A. Foekens; Nils Brünner; H. Offenberg

doi:10.1016/j.molonc.2007.05.002

Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue

P. A. Usher, A. M. Sieuwerts, A. Bartels, U. Lademann, Hans Jørgen Nielsen, L. Holten-Andersen, J. A. Foekens, Nils Brünner, H. Offenberg

Gastroenheden AHH

15 Citationer (Scopus)

Abstract

TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified in tumor tissue, but not in plasma samples obtained from cancer patients.

The two splice variants of TIMP-1 may hold important clinical information, and either alone or in combination with measurement of full-length TIMP-1 they may improve the prognostic and/or predictive value of TIMP-1 analyses.

Udgivelsesdato: september

Originalsprog	Engelsk
Tidsskrift	Molecular Oncology
Vol/bind	1
Udgave nummer	2
Sider (fra-til)	205-15
Antal sider	11
DOI	https://doi.org/10.1016/j.molonc.2007.05.002
Status	Udgivet - 2007

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10.1016/j.molonc.2007.05.002

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B8CWB-4NSMMY7-1&_user=641710&_coverDate=09%2F30%2F2007&_rdoc=13&_fmt=full&_orig=browse&_srch=doc-info(%23toc%2340054%232007%23999989997%23665344%23FLA%23display%23Volume)&_cdi=40054&_sort=d&_docanchor=&_ct=17&_acct=C000034378&_version=1&_urlVersion=0&_userid=641710&md5=353bb086256c178add51d52d215ad030

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title = "Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue",

abstract = "TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified in tumor tissue, but not in plasma samples obtained from cancer patients.The two splice variants of TIMP-1 may hold important clinical information, and either alone or in combination with measurement of full-length TIMP-1 they may improve the prognostic and/or predictive value of TIMP-1 analyses.",

keywords = "TIMP-1, splice variants, colon cancer, cancer cell lines",

author = "Usher, \{P. A.\} and Sieuwerts, \{A. M.\} and A. Bartels and U. Lademann and Nielsen, \{Hans J{\o}rgen\} and L. Holten-Andersen and Foekens, \{J. A.\} and Nils Br{\"u}nner and H. Offenberg",

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doi = "10.1016/j.molonc.2007.05.002",

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T1 - Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue

AU - Usher, P. A.

AU - Sieuwerts, A. M.

AU - Bartels, A.

AU - Lademann, U.

AU - Nielsen, Hans Jørgen

AU - Holten-Andersen, L.

AU - Foekens, J. A.

AU - Brünner, Nils

AU - Offenberg, H.

PY - 2007

Y1 - 2007

N2 - TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified in tumor tissue, but not in plasma samples obtained from cancer patients.The two splice variants of TIMP-1 may hold important clinical information, and either alone or in combination with measurement of full-length TIMP-1 they may improve the prognostic and/or predictive value of TIMP-1 analyses.

AB - TIMP-1 is a promising new candidate as a prognostic marker in colorectal and breast cancer. We now describe the discovery of two alternatively spliced variants of TIMP-1 mRNA. The two variants lacking exon 2 (del-2) and 5 (del-5), respectively, were identified in human cancer cell lines by RT-PCR. The del-2 variant was, furthermore, detected in extracts from 12 colorectal cancer tissue samples. By western blotting additional bands of lower molecular mass than full-length TIMP-1 were identified in tumor tissue, but not in plasma samples obtained from cancer patients.The two splice variants of TIMP-1 may hold important clinical information, and either alone or in combination with measurement of full-length TIMP-1 they may improve the prognostic and/or predictive value of TIMP-1 analyses.

KW - TIMP-1

KW - splice variants

KW - colon cancer

KW - cancer cell lines

UR - https://www.scopus.com/pages/publications/34547573074

U2 - 10.1016/j.molonc.2007.05.002

DO - 10.1016/j.molonc.2007.05.002

M3 - Journal article

SN - 1878-0261

VL - 1

SP - 205

EP - 215

JO - Molecular Oncology

JF - Molecular Oncology

IS - 2

ER -

Identification of alternatively spliced TIMP-1 mRNA in cancer cell lines and colon cancer tissue

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