Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures

P H Jakobsen; P Grellier; T G Theander; N Behrendt; M Torii; M Aikawa; J Schrevel; S Jepsen

Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures

P H Jakobsen, P Grellier, T G Theander, N Behrendt, M Torii, M Aikawa, J Schrevel, S Jepsen

3 Citationer (Scopus)

Abstract

The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.

Originalsprog	Engelsk
Tidsskrift	APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Vol/bind	99
Udgave nummer	2
Sider (fra-til)	155-62
Antal sider	8
ISSN	0903-4641
Status	Udgivet - feb. 1991

Citationsformater

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title = "Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures",

abstract = "The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.",

keywords = "Adult, Animals, Antigens, Protozoan, Counterimmunoelectrophoresis, Humans, Immunoblotting, Plasmodium falciparum, Solubility",

author = "Jakobsen, {P H} and P Grellier and Theander, {T G} and N Behrendt and M Torii and M Aikawa and J Schrevel and S Jepsen",

year = "1991",

month = feb,

language = "English",

volume = "99",

pages = "155--62",

journal = "APMIS : acta pathologica, microbiologica, et immunologica Scandinavica",

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TY - JOUR

T1 - Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures

AU - Jakobsen, P H

AU - Grellier, P

AU - Theander, T G

AU - Behrendt, N

AU - Torii, M

AU - Aikawa, M

AU - Schrevel, J

AU - Jepsen, S

PY - 1991/2

Y1 - 1991/2

N2 - The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.

AB - The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.

KW - Adult

KW - Animals

KW - Antigens, Protozoan

KW - Counterimmunoelectrophoresis

KW - Humans

KW - Immunoblotting

KW - Plasmodium falciparum

KW - Solubility

M3 - Journal article

C2 - 2001282

SN - 0903-4641

VL - 99

SP - 155

EP - 162

JO - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

IS - 2

ER -

Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures

Abstract

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