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Hypophosphatemic Hypovitaminosis D induces Osteomalacia in the adult female rat

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Harvard

Durup, D, Diaz-delCastillo, M, Morgenlykke, J, Jensen, LT, Frandsen, E, Abelson, KSP, Pedersen, L, Lykkesfeldt, J, Ding, M, Jørgensen, NR, Syberg, S, Petersen, S & Heegaard, A-M 2020, 'Hypophosphatemic Hypovitaminosis D induces Osteomalacia in the adult female rat', Endocrinology, bind 161, nr. 8, bqaa100. https://doi.org/10.1210/endocr/bqaa100

APA

Durup, D., Diaz-delCastillo, M., Morgenlykke, J., Jensen, L. T., Frandsen, E., Abelson, K. S. P., Pedersen, L., Lykkesfeldt, J., Ding, M., Jørgensen, N. R., Syberg, S., Petersen, S., & Heegaard, A-M. (2020). Hypophosphatemic Hypovitaminosis D induces Osteomalacia in the adult female rat. Endocrinology, 161(8), [bqaa100]. https://doi.org/10.1210/endocr/bqaa100

CBE

Durup D, Diaz-delCastillo M, Morgenlykke J, Jensen LT, Frandsen E, Abelson KSP, Pedersen L, Lykkesfeldt J, Ding M, Jørgensen NR, Syberg S, Petersen S, Heegaard A-M. 2020. Hypophosphatemic Hypovitaminosis D induces Osteomalacia in the adult female rat. Endocrinology. 161(8):Article bqaa100. https://doi.org/10.1210/endocr/bqaa100

MLA

Vancouver

Author

Durup, Darshana ; Diaz-delCastillo, Marta ; Morgenlykke, Jesper ; Jensen, Lars Thorbjorn ; Frandsen, Erik ; Abelson, Klas S P ; Pedersen, Lars ; Lykkesfeldt, Jens ; Ding, Ming ; Jørgensen, Niklas R ; Syberg, Susanne ; Petersen, Solveig ; Heegaard, Anne-Marie. / Hypophosphatemic Hypovitaminosis D induces Osteomalacia in the adult female rat. I: Endocrinology. 2020 ; Bind 161, Nr. 8.

Bibtex

@article{a29ec9b65c1d4386a25faeb5af9d355c,
title = "Hypophosphatemic Hypovitaminosis D induces Osteomalacia in the adult female rat",
abstract = "Osteomalacia is a bone-demineralizing disease of adulthood, often caused by hypovitaminosis D. Current animal models of the disease mimic osteomalacia as a consequence of gastric bypass or toxic exposure to metals, but a relevant model of diet-induced osteomalacia is lacking. For that purpose, 7-month-old female Sprague Dawley rats were randomly assigned into 2 weight-stratified groups and maintained for 4 months on synthetic diets containing negligible or normal levels of vitamin D. The dietary regimen resulted in vitamin D deficiency as measured by 25-hydroxyvitamin D serum levels; however, hypovitaminosis D per se did not affect biomarkers of calcium metabolism and bone turnover, nor did it result in increased osteoid. Thus, vitamin D depletion through the diet was found to be insufficient to induce an osteomalacia-like phenotype in the adult rat. After 4 months, the phosphate content of the vitamin D-depleted diet had decreased to 0.16% (calcium:phosphorus ratio of 5.85), resulting in an osteomalacic-like condition (trabecular osteoid surface/bone surface constituted 33%; CI, 26-40). The diet change also affected both metabolic and bone turnover biomarkers, including significantly suppressing serum fibroblast growth factor 23. Furthermore, decreased dietary phosphate in a vitamin D-depleted diet led to microarchitectural changes of trabecular and cortical bone, lower bone mass density, lower bone mass content and decreased bone strength, all indicating reduced bone quality. Taken together, our results show that osteomalacia can be induced in the adult female rat by depleting vitamin D and lowering phosphate content in the diet.",
author = "Darshana Durup and Marta Diaz-delCastillo and Jesper Morgenlykke and Jensen, {Lars Thorbjorn} and Erik Frandsen and Abelson, {Klas S P} and Lars Pedersen and Jens Lykkesfeldt and Ming Ding and J{\o}rgensen, {Niklas R} and Susanne Syberg and Solveig Petersen and Anne-Marie Heegaard",
note = "{\textcopyright} Endocrine Society 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.",
year = "2020",
month = aug,
day = "1",
doi = "10.1210/endocr/bqaa100",
language = "English",
volume = "161",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The/Endocrine Society",
number = "8",

}

RIS

TY - JOUR

T1 - Hypophosphatemic Hypovitaminosis D induces Osteomalacia in the adult female rat

AU - Durup, Darshana

AU - Diaz-delCastillo, Marta

AU - Morgenlykke, Jesper

AU - Jensen, Lars Thorbjorn

AU - Frandsen, Erik

AU - Abelson, Klas S P

AU - Pedersen, Lars

AU - Lykkesfeldt, Jens

AU - Ding, Ming

AU - Jørgensen, Niklas R

AU - Syberg, Susanne

AU - Petersen, Solveig

AU - Heegaard, Anne-Marie

N1 - © Endocrine Society 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - Osteomalacia is a bone-demineralizing disease of adulthood, often caused by hypovitaminosis D. Current animal models of the disease mimic osteomalacia as a consequence of gastric bypass or toxic exposure to metals, but a relevant model of diet-induced osteomalacia is lacking. For that purpose, 7-month-old female Sprague Dawley rats were randomly assigned into 2 weight-stratified groups and maintained for 4 months on synthetic diets containing negligible or normal levels of vitamin D. The dietary regimen resulted in vitamin D deficiency as measured by 25-hydroxyvitamin D serum levels; however, hypovitaminosis D per se did not affect biomarkers of calcium metabolism and bone turnover, nor did it result in increased osteoid. Thus, vitamin D depletion through the diet was found to be insufficient to induce an osteomalacia-like phenotype in the adult rat. After 4 months, the phosphate content of the vitamin D-depleted diet had decreased to 0.16% (calcium:phosphorus ratio of 5.85), resulting in an osteomalacic-like condition (trabecular osteoid surface/bone surface constituted 33%; CI, 26-40). The diet change also affected both metabolic and bone turnover biomarkers, including significantly suppressing serum fibroblast growth factor 23. Furthermore, decreased dietary phosphate in a vitamin D-depleted diet led to microarchitectural changes of trabecular and cortical bone, lower bone mass density, lower bone mass content and decreased bone strength, all indicating reduced bone quality. Taken together, our results show that osteomalacia can be induced in the adult female rat by depleting vitamin D and lowering phosphate content in the diet.

AB - Osteomalacia is a bone-demineralizing disease of adulthood, often caused by hypovitaminosis D. Current animal models of the disease mimic osteomalacia as a consequence of gastric bypass or toxic exposure to metals, but a relevant model of diet-induced osteomalacia is lacking. For that purpose, 7-month-old female Sprague Dawley rats were randomly assigned into 2 weight-stratified groups and maintained for 4 months on synthetic diets containing negligible or normal levels of vitamin D. The dietary regimen resulted in vitamin D deficiency as measured by 25-hydroxyvitamin D serum levels; however, hypovitaminosis D per se did not affect biomarkers of calcium metabolism and bone turnover, nor did it result in increased osteoid. Thus, vitamin D depletion through the diet was found to be insufficient to induce an osteomalacia-like phenotype in the adult rat. After 4 months, the phosphate content of the vitamin D-depleted diet had decreased to 0.16% (calcium:phosphorus ratio of 5.85), resulting in an osteomalacic-like condition (trabecular osteoid surface/bone surface constituted 33%; CI, 26-40). The diet change also affected both metabolic and bone turnover biomarkers, including significantly suppressing serum fibroblast growth factor 23. Furthermore, decreased dietary phosphate in a vitamin D-depleted diet led to microarchitectural changes of trabecular and cortical bone, lower bone mass density, lower bone mass content and decreased bone strength, all indicating reduced bone quality. Taken together, our results show that osteomalacia can be induced in the adult female rat by depleting vitamin D and lowering phosphate content in the diet.

U2 - 10.1210/endocr/bqaa100

DO - 10.1210/endocr/bqaa100

M3 - Journal article

C2 - 32591825

VL - 161

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 8

M1 - bqaa100

ER -

ID: 60799350