TY - JOUR
T1 - Human preovulatory follicular fluid
T2 - inhibin and free steroids related to optimal follicular maturation in ovarian stimulation regimes and possible function in ovulation
AU - Andersen, C Y
AU - Westergaard, L G
AU - Sinosich, M J
AU - Byskov, A G
PY - 1992/7
Y1 - 1992/7
N2 - Concentrations of inhibin, total and free oestradiol and progesterone were determined in preovulatory follicular fluid from 15 women undergoing in-vitro fertilization and embryo transfer treatment. The women underwent ovarian stimulation using clomiphene citrate and human menopausal gonadotrophin (HMG) (69 follicular fluid samples) in one cycle, and gonadotrophin-releasing hormone agonist (GnRHa) and HMG stimulation in the next treatment cycle (64 follicular fluid samples). The women thereby served as their own control. Inhibin, total oestradiol and progesterone were measured by radioimmunoassay. Concentrations of free steroid were calculated after quantitation of the steroid binding proteins, i.e. sex-hormone binding globulin (SHBG), cortisol binding protein (CBP) and albumin. Levels of inhibin and free and total progesterone were significantly higher in follicular fluids collected after stimulation with the GnRHa compared to the clomiphene regime (P less than 0.05, P less than 0.001, P less than 0.001, respectively). In contrast, levels of total and free oestradiol in follicular fluid were significantly lower after stimulation with GnRHa than after clomiphene stimulation (P less than 0.001). These results indicate that the follicles have achieved a more optimal maturation during the GnRHa regimen than during the clomiphene regime. It is suggested that the concentration of free biologically active steroids in follicular fluid, released into the peritoneal cavity during ovulation, may be physiologically important in stimulating the oviduct and the uterus in connection with ovulation, pre-embryo development and implantation.
AB - Concentrations of inhibin, total and free oestradiol and progesterone were determined in preovulatory follicular fluid from 15 women undergoing in-vitro fertilization and embryo transfer treatment. The women underwent ovarian stimulation using clomiphene citrate and human menopausal gonadotrophin (HMG) (69 follicular fluid samples) in one cycle, and gonadotrophin-releasing hormone agonist (GnRHa) and HMG stimulation in the next treatment cycle (64 follicular fluid samples). The women thereby served as their own control. Inhibin, total oestradiol and progesterone were measured by radioimmunoassay. Concentrations of free steroid were calculated after quantitation of the steroid binding proteins, i.e. sex-hormone binding globulin (SHBG), cortisol binding protein (CBP) and albumin. Levels of inhibin and free and total progesterone were significantly higher in follicular fluids collected after stimulation with the GnRHa compared to the clomiphene regime (P less than 0.05, P less than 0.001, P less than 0.001, respectively). In contrast, levels of total and free oestradiol in follicular fluid were significantly lower after stimulation with GnRHa than after clomiphene stimulation (P less than 0.001). These results indicate that the follicles have achieved a more optimal maturation during the GnRHa regimen than during the clomiphene regime. It is suggested that the concentration of free biologically active steroids in follicular fluid, released into the peritoneal cavity during ovulation, may be physiologically important in stimulating the oviduct and the uterus in connection with ovulation, pre-embryo development and implantation.
KW - Albumins/metabolism
KW - Buserelin/therapeutic use
KW - Carrier Proteins/metabolism
KW - Clomiphene/therapeutic use
KW - Estradiol/metabolism
KW - Female
KW - Fertilization in Vitro
KW - Follicular Fluid/metabolism
KW - Humans
KW - Inhibins/metabolism
KW - Menotropins/therapeutic use
KW - Ovulation/physiology
KW - Ovulation Induction
KW - Progesterone/metabolism
KW - Sex Hormone-Binding Globulin/metabolism
U2 - 10.1093/oxfordjournals.humrep.a137734
DO - 10.1093/oxfordjournals.humrep.a137734
M3 - Journal article
C2 - 1500472
SN - 0268-1161
VL - 7
SP - 765
EP - 769
JO - Human reproduction (Oxford, England)
JF - Human reproduction (Oxford, England)
IS - 6
ER -