Human osteoclast culture and phenotypic characterization

Ankita Agrawal, James A. Gallagher, Alison Gartland*

*Corresponding author af dette arbejde
17 Citationer (Scopus)

Abstract

Bone remodelling occurs throughout life via the coupled actions of bone resorption and bone formation. When the balance of bone resorption and formation becomes unequal, bone diseases, such as osteoporosis occur, while the absence of functional osteoclasts leads to diseases such as osteopetrosis and pycnodysostosis. In order to develop effective treatments for bone disease the normal regulatory systems involved in bone resorption need to be fully elucidated. The only cell in the body capable of resorbing bone is the osteoclast - a highly specialized cell of haematopoietic origin. Until relatively recently, the ability to study the formation and function of human osteoclasts in vitro has been limited. In this chapter, we provide an up-to-date detailed guide to isolating and culturing primary osteoclasts from human peripheral blood. In addition, we detail the methodology used to characterize osteoclasts and how to quantify resorption in vitro. In combination these methods provide a powerful tool in the study of human osteoclasts and the development of new novel treatments for bone disease.

OriginalsprogEngelsk
TitelMethods in Molecular Biology
RedaktørerRagai Mitry, Robin Hughes
Antal sider19
ForlagSpringer Science $ Business Media
Publikationsdato2012
Sider357-375
ISBN (Trykt)9781617793660
DOI
StatusUdgivet - 2012
NavnMethods in Molecular Biology
Vol/bind806
ISSN1064-3745

Fingeraftryk

Dyk ned i forskningsemnerne om 'Human osteoclast culture and phenotypic characterization'. Sammen danner de et unikt fingeraftryk.

Citationsformater