Histidine buffered media maintains pH stabile during cooled transportation of human ovarian tissue

Susanne Elisabeth Pors*, Stine Gry Kristensen, Dmitry Nikiforov, Linn Salto Mamsen, Jesus Cadenas, Vinnie Hornshøj Greve, Margit Dueholm, Claus Yding Andersen

*Corresponding author af dette arbejde
    2 Citationer (Scopus)

    Abstract

    The aim of this study was to investigate whether pH is stable when transporting ovarian tissue in media buffered with either HEPES or histidine. Furthermore, if the choice of transport media impacts the in vitro maturation rate of oocytes collected in connection with ovarian tissue cryopreservation. Human ovaries (n = 34) collected for ovarian tissue cryopreservation were transported immersed in either 30 ml of HEPES buffered (follicle flushing media (Origio; Denmark)) or histidine buffered media (Custodiol®-HTK, Koehler-Chemie, Germany). Tissue was transported on ice for 4-5 h. At arrival, the ovary was weighed, and the pH of the media was measured at 0 °C. From 15 patients, immature oocytes were collected for in vitro maturation, oocytes that matured to metaphase II were evaluated. The pH measured in the HEPES buffered media (pH = 7.5 ± 0.13, n = 18) was significantly higher (p < 0.001) than the pH measured in the histidine buffered media (pH = 7.2 ± 0.05, n = 16). The standard deviation of pH measurements for the histidine buffered media was significantly lower than for the HEPES buffered media measurements (p < 0.0001). A total of 170 and 247 immature oocytes were collected and in vitro matured from ovaries transported in HEPES and histidine buffered media, respectively. The maturation rate of immature oocytes after IVM was similar in the two groups. The results show that pH in the histidine buffered media is closer to the physiological level and more stable than in HEPES buffered medium and support the use of histidine buffered media for cooled transportation of human ovaries.

    OriginalsprogEngelsk
    Artikelnummer116
    TidsskriftJournal of Ovarian Research
    Vol/bind14
    Udgave nummer1
    Sider (fra-til)116
    ISSN1757-2215
    DOI
    StatusUdgivet - 3 sep. 2021

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