Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

High-Titer Hepatitis C Virus Production in a Scalable Single-Use High Cell Density Bioreactor

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

DOI

  1. Neutralizing Antibodies Against Hepatitis C Virus and Their Role in Vaccine Immunity

    Publikation: Bidrag til tidsskriftLederpeer review

  2. Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and Replication

    Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

Vis graf over relationer

Hepatitis C virus (HCV) infections pose a major public health burden due to high chronicity rates and associated morbidity and mortality. A vaccine protecting against chronic infection is not available but would be important for global control of HCV infections. In this study, cell culture-based HCV production was established in a packed-bed bioreactor (CelCradle™) aiming to further the development of an inactivated whole virus vaccine and to facilitate virological and immunological studies requiring large quantities of virus particles. HCV was produced in human hepatoma-derived Huh7.5 cells maintained in serum-free medium on days of virus harvesting. Highest virus yields were obtained when the culture was maintained with two medium exchanges per day. However, increasing the total number of cells in the culture vessel negatively impacted infectivity titers. Peak infectivity titers of up to 7.2 log10 focus forming units (FFU)/mL, accumulated virus yields of up to 5.9 × 1010 FFU, and a cell specific virus yield of up to 41 FFU/cell were obtained from one CelCradle™. CelCradle™-derived and T flask-derived virus had similar characteristics regarding neutralization sensitivity and buoyant density. This packed-bed tide-motion system is available with larger vessels and may thus be a promising platform for large-scale HCV production.

OriginalsprogEngelsk
Artikelnummer249
TidsskriftVaccines
Vol/bind10
Udgave nummer2
Sider (fra-til)1-23
Antal sider23
ISSN2076-393X
DOI
StatusUdgivet - 7 feb. 2022

ID: 75594289