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High-resolution in situ transcriptomics of Pseudomonas aeruginosa unveils genotype independent patho-phenotypes in cystic fibrosis lungs

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@article{8e4f78b155bc445a8e09896e6eea5e57,
title = "High-resolution in situ transcriptomics of Pseudomonas aeruginosa unveils genotype independent patho-phenotypes in cystic fibrosis lungs",
abstract = "Life-long bacterial infections in cystic fibrosis (CF) airways constitute an excellent model both for persistent infections and for microbial adaptive evolution in complex dynamic environments. Using high-resolution transcriptomics applied on CF sputum, we profile transcriptional phenotypes of Pseudomonas aeruginosa populations in patho-physiological conditions. Here we show that the soft-core genome of genetically distinct populations, while maintaining transcriptional flexibility, shares a common expression program tied to the lungs environment. We identify genetically independent traits defining P. aeruginosa physiology in vivo, documenting the connection between several previously identified mutations in CF isolates and some of the convergent phenotypes known to develop in later stages of the infection. In addition, our data highlight to what extent this organism can exploit its extensive repertoire of physiological pathways to acclimate to a new niche and suggest how alternative nutrients produced in the lungs may be utilized in unexpected metabolic contexts.",
keywords = "Adult, Cystic Fibrosis/genetics, Female, Genotype, Humans, Lung/metabolism, Male, Middle Aged, Mutation/genetics, Phenotype, Pseudomonas aeruginosa/genetics, Transcriptome/genetics, Whole Genome Sequencing/methods",
author = "Elio Rossi and Marilena Falcone and S{\o}ren Molin and Johansen, {Helle Krogh}",
year = "2018",
month = "8",
day = "27",
doi = "10.1038/s41467-018-05944-5",
language = "English",
volume = "9",
pages = "3459",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - High-resolution in situ transcriptomics of Pseudomonas aeruginosa unveils genotype independent patho-phenotypes in cystic fibrosis lungs

AU - Rossi, Elio

AU - Falcone, Marilena

AU - Molin, Søren

AU - Johansen, Helle Krogh

PY - 2018/8/27

Y1 - 2018/8/27

N2 - Life-long bacterial infections in cystic fibrosis (CF) airways constitute an excellent model both for persistent infections and for microbial adaptive evolution in complex dynamic environments. Using high-resolution transcriptomics applied on CF sputum, we profile transcriptional phenotypes of Pseudomonas aeruginosa populations in patho-physiological conditions. Here we show that the soft-core genome of genetically distinct populations, while maintaining transcriptional flexibility, shares a common expression program tied to the lungs environment. We identify genetically independent traits defining P. aeruginosa physiology in vivo, documenting the connection between several previously identified mutations in CF isolates and some of the convergent phenotypes known to develop in later stages of the infection. In addition, our data highlight to what extent this organism can exploit its extensive repertoire of physiological pathways to acclimate to a new niche and suggest how alternative nutrients produced in the lungs may be utilized in unexpected metabolic contexts.

AB - Life-long bacterial infections in cystic fibrosis (CF) airways constitute an excellent model both for persistent infections and for microbial adaptive evolution in complex dynamic environments. Using high-resolution transcriptomics applied on CF sputum, we profile transcriptional phenotypes of Pseudomonas aeruginosa populations in patho-physiological conditions. Here we show that the soft-core genome of genetically distinct populations, while maintaining transcriptional flexibility, shares a common expression program tied to the lungs environment. We identify genetically independent traits defining P. aeruginosa physiology in vivo, documenting the connection between several previously identified mutations in CF isolates and some of the convergent phenotypes known to develop in later stages of the infection. In addition, our data highlight to what extent this organism can exploit its extensive repertoire of physiological pathways to acclimate to a new niche and suggest how alternative nutrients produced in the lungs may be utilized in unexpected metabolic contexts.

KW - Adult

KW - Cystic Fibrosis/genetics

KW - Female

KW - Genotype

KW - Humans

KW - Lung/metabolism

KW - Male

KW - Middle Aged

KW - Mutation/genetics

KW - Phenotype

KW - Pseudomonas aeruginosa/genetics

KW - Transcriptome/genetics

KW - Whole Genome Sequencing/methods

U2 - 10.1038/s41467-018-05944-5

DO - 10.1038/s41467-018-05944-5

M3 - Journal article

VL - 9

SP - 3459

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -

ID: 56201445