TY - JOUR
T1 - Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia
AU - Rosenberg, Tine
AU - Aaberg-Jessen, Charlotte
AU - Petterson, Stine Asferg
AU - Kristensen, Bjarne Winther
PY - 2018/4
Y1 - 2018/4
N2 - AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.
AB - AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.
KW - AC133 Antigen/metabolism
KW - Antigens, Neoplasm/metabolism
KW - Biomarkers, Tumor/metabolism
KW - Brain Neoplasms/metabolism
KW - Carbonic Anhydrase IX/metabolism
KW - Cell Hypoxia/physiology
KW - Cell Proliferation/physiology
KW - Gene Expression Regulation, Neoplastic
KW - Glioblastoma/metabolism
KW - Humans
KW - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
KW - Ki-67 Antigen/metabolism
KW - Neoplastic Stem Cells/metabolism
KW - Nestin/metabolism
KW - Spheroids, Cellular/metabolism
KW - Tumor Cells, Cultured
KW - Vascular Endothelial Growth Factor A/metabolism
U2 - 10.2217/cns-2017-0034
DO - 10.2217/cns-2017-0034
M3 - Journal article
C2 - 29708435
SN - 2045-0907
VL - 7
SP - CNS15
JO - CNS oncology
JF - CNS oncology
IS - 2
ER -