Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Heterogeneity in the perirenal region of humans suggests presence of dormant brown adipose tissue that contains brown fat precursor cells

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. The aromatic amino acid sensor GPR142 controls metabolism through balanced regulation of pancreatic and gut hormones

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Exercise-induced molecular mechanisms promoting glycogen supercompensation in human skeletal muscle

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. The role of IL-1 in postprandial fatigue

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Enterochromaffin 5-HT cells - A major target for GLP-1 and gut microbial metabolites

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Epicardial and pericardial adipose tissues are associated with reduced diastolic and systolic function in type 2 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. IL-6 release from muscles during exercise is stimulated by lactate-dependent protease activity

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Exercise-mediated improvement of depression in patients with gastro-esophageal junction cancer is linked to kynurenine metabolism

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Markers of bone turnover are reduced in patients with CF related diabetes; the role of glucose

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

OBJECTIVE: Increasing the amounts of functionally competent brown adipose tissue (BAT) in adult humans has the potential to restore dysfunctional metabolism and counteract obesity. In this study, we aimed to characterize the human perirenal fat depot, and we hypothesized that there would be regional, within-depot differences in the adipose signature depending on local sympathetic activity.

METHODS: We characterized fat specimens from four different perirenal regions of adult kidney donors, through a combination of qPCR mapping, immunohistochemical staining, RNA-sequencing, and pre-adipocyte isolation. Candidate gene signatures, separated by adipocyte morphology, were recapitulated in a murine model of unilocular brown fat induced by thermoneutrality and high fat diet.

RESULTS: We identified widespread amounts of dormant brown adipose tissue throughout the perirenal depot, which was contrasted by multilocular BAT, primarily found near the adrenal gland. Dormant BAT was characterized by a unilocular morphology and a distinct gene expression profile, which partly overlapped with that of subcutaneous white adipose tissue (WAT). Brown fat precursor cells, which differentiated into functional brown adipocytes were present in the entire perirenal fat depot, regardless of state. We identified SPARC as a candidate adipokine contributing to a dormant BAT state, and CLSTN3 as a novel marker for multilocular BAT.

CONCLUSIONS: We propose that perirenal adipose tissue in adult humans consists mainly of dormant BAT and provide a data set for future research on factors which can reactivate dormant BAT into active BAT, a potential strategy for combatting obesity and metabolic disease.

OriginalsprogEngelsk
TidsskriftMolecular Metabolism
Vol/bind24
Sider (fra-til)30-43
Antal sider14
ISSN2212-8778
DOI
StatusUdgivet - jun. 2019

Bibliografisk note

Copyright © 2019 The Authors. Published by Elsevier GmbH.. All rights reserved.

ID: 58147153