Hepatitis C virus RNA functionally sequesters miR-122

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Region Hovedstaden - en del af Københavns Universitetshospital

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Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Luna, JM, Scheel, TKH, Danino, T, Shaw, KS, Mele, A, Fak, JJ, Nishiuchi, E, Takacs, CN, Catanese, MT, de Jong, YP, Jacobson, IM, Rice, CM & Darnell, RB 2015, 'Hepatitis C virus RNA functionally sequesters miR-122' Cell, bind 160, nr. 6, s. 1099-110. https://doi.org/10.1016/j.cell.2015.02.025

APA

Luna, J. M., Scheel, T. K. H., Danino, T., Shaw, K. S., Mele, A., Fak, J. J., ... Darnell, R. B. (2015). Hepatitis C virus RNA functionally sequesters miR-122. Cell, 160(6), 1099-110. https://doi.org/10.1016/j.cell.2015.02.025

CBE

Luna JM, Scheel TKH, Danino T, Shaw KS, Mele A, Fak JJ, Nishiuchi E, Takacs CN, Catanese MT, de Jong YP, Jacobson IM, Rice CM, Darnell RB. 2015. Hepatitis C virus RNA functionally sequesters miR-122. Cell. 160(6):1099-110. https://doi.org/10.1016/j.cell.2015.02.025

MLA

Luna, Joseph M o.a.. "Hepatitis C virus RNA functionally sequesters miR-122". Cell. 2015, 160(6). 1099-110. https://doi.org/10.1016/j.cell.2015.02.025

Vancouver

Luna JM, Scheel TKH, Danino T, Shaw KS, Mele A, Fak JJ o.a. Hepatitis C virus RNA functionally sequesters miR-122. Cell. 2015 mar 12;160(6):1099-110. https://doi.org/10.1016/j.cell.2015.02.025

Author

Luna, Joseph M ; Scheel, Troels K H ; Danino, Tal ; Shaw, Katharina S ; Mele, Aldo ; Fak, John J ; Nishiuchi, Eiko ; Takacs, Constantin N ; Catanese, Maria Teresa ; de Jong, Ype P ; Jacobson, Ira M ; Rice, Charles M ; Darnell, Robert B. / Hepatitis C virus RNA functionally sequesters miR-122. I: Cell. 2015 ; Bind 160, Nr. 6. s. 1099-110.

Bibtex

@article{e04b70d1407346eea4492eec7330bff7,

title = "Hepatitis C virus RNA functionally sequesters miR-122",

abstract = "Hepatitis C virus (HCV) uniquely requires the liver-specific microRNA-122 for replication, yet global effects on endogenous miRNA targets during infection are unexplored. Here, high-throughput sequencing and crosslinking immunoprecipitation (HITS-CLIP) experiments of human Argonaute (AGO) during HCV infection showed robust AGO binding on the HCV 5'UTR at known and predicted miR-122 sites. On the human transcriptome, we observed reduced AGO binding and functional mRNA de-repression of miR-122 targets during virus infection. This miR-122 {"}sponge{"} effect was relieved and redirected to miR-15 targets by swapping the miRNA tropism of the virus. Single-cell expression data from reporters containing miR-122 sites showed significant de-repression during HCV infection depending on expression level and site number. We describe a quantitative mathematical model of HCV-induced miR-122 sequestration and propose that such miR-122 inhibition by HCV RNA may result in global de-repression of host miR-122 targets, providing an environment fertile for the long-term oncogenic potential of HCV.",

keywords = "Argonaute Proteins, Base Sequence, Cell Line, Tumor, Eukaryotic Initiation Factors, Hepacivirus, Hepatitis C, Humans, Liver, MicroRNAs, Molecular Sequence Data, RNA, Viral, Virus Replication",

author = "Luna, {Joseph M} and Scheel, {Troels K H} and Tal Danino and Shaw, {Katharina S} and Aldo Mele and Fak, {John J} and Eiko Nishiuchi and Takacs, {Constantin N} and Catanese, {Maria Teresa} and {de Jong}, {Ype P} and Jacobson, {Ira M} and Rice, {Charles M} and Darnell, {Robert B}",

year = "2015",

month = "3",

day = "12",

doi = "10.1016/j.cell.2015.02.025",

language = "English",

volume = "160",

pages = "1099--110",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "6",

}

RIS

TY - JOUR

T1 - Hepatitis C virus RNA functionally sequesters miR-122

AU - Luna, Joseph M

AU - Scheel, Troels K H

AU - Danino, Tal

AU - Shaw, Katharina S

AU - Mele, Aldo

AU - Fak, John J

AU - Nishiuchi, Eiko

AU - Takacs, Constantin N

AU - Catanese, Maria Teresa

AU - de Jong, Ype P

AU - Jacobson, Ira M

AU - Rice, Charles M

AU - Darnell, Robert B

PY - 2015/3/12

Y1 - 2015/3/12

N2 - Hepatitis C virus (HCV) uniquely requires the liver-specific microRNA-122 for replication, yet global effects on endogenous miRNA targets during infection are unexplored. Here, high-throughput sequencing and crosslinking immunoprecipitation (HITS-CLIP) experiments of human Argonaute (AGO) during HCV infection showed robust AGO binding on the HCV 5'UTR at known and predicted miR-122 sites. On the human transcriptome, we observed reduced AGO binding and functional mRNA de-repression of miR-122 targets during virus infection. This miR-122 "sponge" effect was relieved and redirected to miR-15 targets by swapping the miRNA tropism of the virus. Single-cell expression data from reporters containing miR-122 sites showed significant de-repression during HCV infection depending on expression level and site number. We describe a quantitative mathematical model of HCV-induced miR-122 sequestration and propose that such miR-122 inhibition by HCV RNA may result in global de-repression of host miR-122 targets, providing an environment fertile for the long-term oncogenic potential of HCV.

AB - Hepatitis C virus (HCV) uniquely requires the liver-specific microRNA-122 for replication, yet global effects on endogenous miRNA targets during infection are unexplored. Here, high-throughput sequencing and crosslinking immunoprecipitation (HITS-CLIP) experiments of human Argonaute (AGO) during HCV infection showed robust AGO binding on the HCV 5'UTR at known and predicted miR-122 sites. On the human transcriptome, we observed reduced AGO binding and functional mRNA de-repression of miR-122 targets during virus infection. This miR-122 "sponge" effect was relieved and redirected to miR-15 targets by swapping the miRNA tropism of the virus. Single-cell expression data from reporters containing miR-122 sites showed significant de-repression during HCV infection depending on expression level and site number. We describe a quantitative mathematical model of HCV-induced miR-122 sequestration and propose that such miR-122 inhibition by HCV RNA may result in global de-repression of host miR-122 targets, providing an environment fertile for the long-term oncogenic potential of HCV.

KW - Argonaute Proteins

KW - Base Sequence

KW - Cell Line, Tumor

KW - Eukaryotic Initiation Factors

KW - Hepacivirus

KW - Hepatitis C

KW - Humans

KW - Liver

KW - MicroRNAs

KW - Molecular Sequence Data

KW - RNA, Viral

KW - Virus Replication

U2 - 10.1016/j.cell.2015.02.025

DO - 10.1016/j.cell.2015.02.025

M3 - Journal article

VL - 160

SP - 1099

EP - 1110

JO - Cell

JF - Cell

SN - 0092-8674

IS - 6

ER -

ID: 45565808