Hb Kalundborg [β79(EF3)Asp→Glu; HBB: c.240C>a], a Possible Low-affinity Hemoglobin Variant Detected during Hb A1c Measurement

Emil B Ahmad-Nielsen, Pal B Szecsi, Palle S Bratholm, Jesper Petersen, Andreas Glenthøj*

*Corresponding author af dette arbejde

Abstrakt

A previously unknown hemoglobin (Hb) variant was detected during measurement of glycosylated Hb (Hb A1c) after the introduction of a new high performance liquid chromatography (HPLC) apparatus. Subsequent DNA sequencing revealed a heterozygous single nucleotide substitution at codon 79 (C>A) on the β-globin gene changing an amino acid [β79(EF3)Asp→Glu; HBB: c.240C>A]. The new Hb variant was named Hb Kalundborg after the place of origin of the proband. Heterozygosity for this mutation appears to have no clinical significance in itself except for a possibly slightly lower oxygen affinity. However, it interferes with Hb A1c measurement by HPLC, causing a falsely high Hb A1c concentration when using the G11 apparatus with clinical implications possibly to follow.

OriginalsprogEngelsk
TidsskriftHemoglobin
Vol/bind46
Udgave nummer2
Sider (fra-til)124-128
Antal sider5
ISSN0363-0269
DOI
StatusUdgivet - mar. 2022

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