TY - JOUR
T1 - Group A Streptococcal DNase Sda1 Impairs Plasmacytoid Dendritic Cells’ Type 1 Interferon Response
AU - Keller, Nadia
AU - Woytschak, Janine
AU - Martin Heeb, Lukas Erwin
AU - Maggio, Ewerton Marques
AU - Shambat, Srikanth Mairpady
AU - Snäll, Johanna
AU - Hyldegaard, Ole
AU - Boyman, Onur
AU - Norrby-Teglund, Anna
AU - Zinkernagel, Annelies Sophie
N1 - Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2019/6
Y1 - 2019/6
N2 - Group A Streptococcus causes severe invasive infections, including necrotizing fasciitis. The expression of an array of virulence factors targeting specific host immune functions impedes successful bacterial clearance. The virulence factor streptococcal DNase Sda1 was previously shown to interfere with the entrapment of bacteria through neutrophil extracellular traps and TLR9 signaling. In this study, we showed that plasmacytoid dendritic cells are recruited to the infected tissue during group A streptococcal necrotizing fasciitis. We found that the streptococcal DNase Sda1 impairs plasmacytoid dendritic cell recruitment by reducing IFN-1 levels at the site of infection. We found that streptococcal DNase Sda1 interferes with stabilization of the DNA by the host molecule HMGB1 protein, which may account for decreased IFN-1 levels at the site of infection.
AB - Group A Streptococcus causes severe invasive infections, including necrotizing fasciitis. The expression of an array of virulence factors targeting specific host immune functions impedes successful bacterial clearance. The virulence factor streptococcal DNase Sda1 was previously shown to interfere with the entrapment of bacteria through neutrophil extracellular traps and TLR9 signaling. In this study, we showed that plasmacytoid dendritic cells are recruited to the infected tissue during group A streptococcal necrotizing fasciitis. We found that the streptococcal DNase Sda1 impairs plasmacytoid dendritic cell recruitment by reducing IFN-1 levels at the site of infection. We found that streptococcal DNase Sda1 interferes with stabilization of the DNA by the host molecule HMGB1 protein, which may account for decreased IFN-1 levels at the site of infection.
U2 - 10.1016/j.jid.2018.11.027
DO - 10.1016/j.jid.2018.11.027
M3 - Journal article
C2 - 30543898
SN - 0022-202X
VL - 139
SP - 1284
EP - 1293
JO - The Journal of investigative dermatology
JF - The Journal of investigative dermatology
IS - 6
ER -