Good performance of an immunoassay based method for nevirapine measurements in human breast milk

Kirsten Salado-Rasmussen; Zahra Persson Theilgaard; Mercy Chiduo; Court Pedersen; Jan Gerstoft; Terese Lea Katzenstein

doi:10.1515/CCLM.2011.184

Good performance of an immunoassay based method for nevirapine measurements in human breast milk

Kirsten Salado-Rasmussen, Zahra Persson Theilgaard, Mercy Chiduo, Court Pedersen, Jan Gerstoft, Terese Lea Katzenstein

4 Citationer (Scopus)

Abstract

Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 μL) and no pre-treatment of the samples.

Originalsprog	Engelsk
Tidsskrift	Clinical Chemistry and Laboratory Medicine
Vol/bind	49
Udgave nummer	7
Sider (fra-til)	1171-5
Antal sider	5
ISSN	1434-6621
DOI	https://doi.org/10.1515/CCLM.2011.184
Status	Udgivet - 1 jul. 2011

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10.1515/CCLM.2011.184

Citationsformater

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title = "Good performance of an immunoassay based method for nevirapine measurements in human breast milk",

abstract = "Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 μL) and no pre-treatment of the samples.",

keywords = "Calibration, Female, Humans, Immunoassay, Limit of Detection, Milk, Human, Nevirapine, Quality Control, Reproducibility of Results",

author = "Kirsten Salado-Rasmussen and {Persson Theilgaard}, Zahra and Mercy Chiduo and Court Pedersen and Jan Gerstoft and Katzenstein, {Terese Lea}",

year = "2011",

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T1 - Good performance of an immunoassay based method for nevirapine measurements in human breast milk

AU - Salado-Rasmussen, Kirsten

AU - Persson Theilgaard, Zahra

AU - Chiduo, Mercy

AU - Pedersen, Court

AU - Gerstoft, Jan

AU - Katzenstein, Terese Lea

PY - 2011/7/1

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N2 - Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 μL) and no pre-treatment of the samples.

AB - Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 μL) and no pre-treatment of the samples.

KW - Calibration

KW - Female

KW - Humans

KW - Immunoassay

KW - Limit of Detection

KW - Milk, Human

KW - Nevirapine

KW - Quality Control

KW - Reproducibility of Results

U2 - 10.1515/CCLM.2011.184

DO - 10.1515/CCLM.2011.184

M3 - Journal article

C2 - 21504374

SN - 1434-6621

VL - 49

SP - 1171

EP - 1175

JO - Clinical Chemistry and Laboratory Medicine

JF - Clinical Chemistry and Laboratory Medicine

IS - 7

ER -

Good performance of an immunoassay based method for nevirapine measurements in human breast milk

Abstract

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