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Udgivet

Gonocyte transformation in congenital undescended testes: what is the role of inhibin-B in cell death?

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. The relation of germ cells per tubule in testes, serum inhibin B and FSH in cryptorchid boys

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  2. Is infertility after surgery for cryptorchidism congenital or acquired?

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  3. The incidence of maldescended testes in Denmark

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  1. Laparoscopy to Assist Surgical Decisions Related to Necrotizing Enterocolitis in Preterm Neonates

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  2. Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys

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  3. Propagation of Spermatogonial Stem Cell-Like Cells From Infant Boys

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  4. Cryptorchidism, gonocyte development, and the risks of germ cell malignancy and infertility: A systematic review

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Vis graf over relationer

PURPOSE: Undescended testes (UDT) are subjected to heat stress, which can disturb gonocyte transformation as well as apoptosis. This study aims to describe the apoptosis pathway occurring during minipuberty of children with unilateral (UDT), and to investigate the role of inhibin-B.

METHODS: Testicular biopsies at unilateral orchidopexy of 10 boys (6-9 months old) with normal inhibin-B (n = 5) or low inhibin-B (n = 5) were selected for immunohistochemistry and TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labelling) assay. Testicular tubules were labelled with antibodies against Anti-Müllerian hormone (AMH, Sertoli cell marker), mouse Vasa Homolog (MVH) and placental alkaline phosphatase (PLAP) (both germ cell markers), cleaved caspase3 (apoptotic marker), and followed by confocal imaging and cell counting with Fiji/ImageJ. Data were analyzed with GraphPad Prism.

RESULTS: In males with low and normal inhibin-B, there was no statistical difference (p > 0.05) in the percentage of testicular tubules containing TUNEL + cells, number of cleaved caspase3 ± germ cells/tubule, total number of germ cells/tubule, and the percentage of fibrotic tubules or number of Sertoli cells/tubule.

CONCLUSIONS: These results suggest that inhibin-B does not regulate cell death of gonocytes and further studies are required to uncover any role of inhibin-B in gonocyte transformation.

OriginalsprogEngelsk
TidsskriftPediatric Surgery International
Vol/bind35
Udgave nummer11
Sider (fra-til)1309-1316
Antal sider8
ISSN0179-0358
DOI
StatusUdgivet - nov. 2019

ID: 57847717