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Gluten-free diet during pregnancy alleviates signs of diabetes and celiac disease in NOD mouse offspring

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@article{eeaf674055014fe7981440c98a50c60a,
title = "Gluten-free diet during pregnancy alleviates signs of diabetes and celiac disease in NOD mouse offspring",
abstract = "BACKGROUND: Gluten-free (GF) diet during pregnancy ameliorates autoimmune diabetes in nonobese diabetic (NOD) mouse offspring. Due to comorbidity of celiac disease in type 1 diabetes, we hypothesized that GF diet in utero alleviates the humoral and histopathological signs of celiac disease in NOD mice. We aimed to establish the mechanisms behind the diabetes-protective effect of GF diet in utero.METHODS: Breeding pairs of NOD mice were fed a GF or gluten-containing standard (STD) diet until parturition. The offspring were nursed by mothers on STD diet and continued on this diet until ages 4 and 13 weeks. Analyses of serum antitissue transglutaminase (anti-tTG) intestine and islet histology, islet transglutaminase (TG) activity, and cytokine expression in T cells from lymphoid organs were performed.RESULTS: GF versus STD diet in utero led to reduced serum anti-tTG titre and increased villus-to-crypt ratio at both ages. Insulitis along with systemic and local inflammation were decreased, but islet TG activity was unchanged in 13-week-old GF mice. These mice had unchanged beta-cell volumes, but increased islet numbers throughout the prediabetic period.CONCLUSIONS: Collectively, GF diet administered during pregnancy improves signs of celiac disease and autoimmune diabetes in the offspring. The diabetes-ameliorative effect of GF diet in utero is followed by dampening of inflammation, unchanged beta-cell volume, but increased islet numbers.",
keywords = "Animals, Animals, Newborn, Biomarkers/analysis, Celiac Disease/diet therapy, Diabetes Mellitus, Experimental/diet therapy, Diet, Gluten-Free, Female, Insulin-Secreting Cells/cytology, Mice, Mice, Inbred NOD, Pregnancy, Prognosis",
author = "Martin Haupt-Jorgensen and Jesper Larsen and Knud Josefsen and J{\o}rgensen, {Tina Z} and Antvorskov, {Julie Christine} and Hansen, {Axel K} and Karsten Buschard",
note = "Copyright {\circledC} 2018 John Wiley & Sons, Ltd.",
year = "2018",
month = "5",
doi = "10.1002/dmrr.2987",
language = "English",
volume = "34",
pages = "e2987",
journal = "Diabetes - Metabolism: Research and Reviews (Print Edition)",
issn = "1520-7552",
publisher = "John/Wiley & Sons Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Gluten-free diet during pregnancy alleviates signs of diabetes and celiac disease in NOD mouse offspring

AU - Haupt-Jorgensen, Martin

AU - Larsen, Jesper

AU - Josefsen, Knud

AU - Jørgensen, Tina Z

AU - Antvorskov, Julie Christine

AU - Hansen, Axel K

AU - Buschard, Karsten

N1 - Copyright © 2018 John Wiley & Sons, Ltd.

PY - 2018/5

Y1 - 2018/5

N2 - BACKGROUND: Gluten-free (GF) diet during pregnancy ameliorates autoimmune diabetes in nonobese diabetic (NOD) mouse offspring. Due to comorbidity of celiac disease in type 1 diabetes, we hypothesized that GF diet in utero alleviates the humoral and histopathological signs of celiac disease in NOD mice. We aimed to establish the mechanisms behind the diabetes-protective effect of GF diet in utero.METHODS: Breeding pairs of NOD mice were fed a GF or gluten-containing standard (STD) diet until parturition. The offspring were nursed by mothers on STD diet and continued on this diet until ages 4 and 13 weeks. Analyses of serum antitissue transglutaminase (anti-tTG) intestine and islet histology, islet transglutaminase (TG) activity, and cytokine expression in T cells from lymphoid organs were performed.RESULTS: GF versus STD diet in utero led to reduced serum anti-tTG titre and increased villus-to-crypt ratio at both ages. Insulitis along with systemic and local inflammation were decreased, but islet TG activity was unchanged in 13-week-old GF mice. These mice had unchanged beta-cell volumes, but increased islet numbers throughout the prediabetic period.CONCLUSIONS: Collectively, GF diet administered during pregnancy improves signs of celiac disease and autoimmune diabetes in the offspring. The diabetes-ameliorative effect of GF diet in utero is followed by dampening of inflammation, unchanged beta-cell volume, but increased islet numbers.

AB - BACKGROUND: Gluten-free (GF) diet during pregnancy ameliorates autoimmune diabetes in nonobese diabetic (NOD) mouse offspring. Due to comorbidity of celiac disease in type 1 diabetes, we hypothesized that GF diet in utero alleviates the humoral and histopathological signs of celiac disease in NOD mice. We aimed to establish the mechanisms behind the diabetes-protective effect of GF diet in utero.METHODS: Breeding pairs of NOD mice were fed a GF or gluten-containing standard (STD) diet until parturition. The offspring were nursed by mothers on STD diet and continued on this diet until ages 4 and 13 weeks. Analyses of serum antitissue transglutaminase (anti-tTG) intestine and islet histology, islet transglutaminase (TG) activity, and cytokine expression in T cells from lymphoid organs were performed.RESULTS: GF versus STD diet in utero led to reduced serum anti-tTG titre and increased villus-to-crypt ratio at both ages. Insulitis along with systemic and local inflammation were decreased, but islet TG activity was unchanged in 13-week-old GF mice. These mice had unchanged beta-cell volumes, but increased islet numbers throughout the prediabetic period.CONCLUSIONS: Collectively, GF diet administered during pregnancy improves signs of celiac disease and autoimmune diabetes in the offspring. The diabetes-ameliorative effect of GF diet in utero is followed by dampening of inflammation, unchanged beta-cell volume, but increased islet numbers.

KW - Animals

KW - Animals, Newborn

KW - Biomarkers/analysis

KW - Celiac Disease/diet therapy

KW - Diabetes Mellitus, Experimental/diet therapy

KW - Diet, Gluten-Free

KW - Female

KW - Insulin-Secreting Cells/cytology

KW - Mice

KW - Mice, Inbred NOD

KW - Pregnancy

KW - Prognosis

U2 - 10.1002/dmrr.2987

DO - 10.1002/dmrr.2987

M3 - Journal article

VL - 34

SP - e2987

JO - Diabetes - Metabolism: Research and Reviews (Print Edition)

JF - Diabetes - Metabolism: Research and Reviews (Print Edition)

SN - 1520-7552

IS - 4

ER -

ID: 56441350