Glutathione depletion inhibits IL-1 beta-stimulated nitric oxide production by reducing inducible nitric oxide synthase gene expression

M A Nikulina; H U Andersen; A E Karlsen; M I Darville; Decio Laks Eizirik; T Mandrup-Poulsen

doi:10.1006/cyto.2000.0712

Glutathione depletion inhibits IL-1 beta-stimulated nitric oxide production by reducing inducible nitric oxide synthase gene expression

M A Nikulina
, H U Andersen
, A E Karlsen
, M I Darville
, Decio Laks Eizirik
, T Mandrup-Poulsen

20 Citationer (Scopus)

Abstract

L-buthionine-S,R-sulfoximine (BSO), an inhibitor of GSH synthesis, decreased IL-1 beta-induced nitrite release in rat islets and purified rat beta cells, nitrite formation and iNOS gene promoter activity in insulinoma cells, and iNOS mRNA expression in rat islets. The thiol depletor diethyl maleate (DEM) and an inhibitor of glutathione reductase 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) reduced IL-1 beta-stimulated nitrite release in islets. We conclude that GSH regulates IL-1 beta-induced NO production in islets, purified beta cells and insulinoma cells by modulation of iNOS gene expression.

Originalsprog	Engelsk
Tidsskrift	Cytokine
Vol/bind	12
Udgave nummer	9
Sider (fra-til)	1391-4
Antal sider	4
ISSN	1043-4666
DOI	https://doi.org/10.1006/cyto.2000.0712
Status	Udgivet - sep. 2000
Udgivet eksternt	Ja

Adgang til dokumentet

10.1006/cyto.2000.0712

Citationsformater

@article{cc75cefb091e4337b73d8ac22518211e,

title = "Glutathione depletion inhibits IL-1 beta-stimulated nitric oxide production by reducing inducible nitric oxide synthase gene expression",

abstract = "L-buthionine-S,R-sulfoximine (BSO), an inhibitor of GSH synthesis, decreased IL-1 beta-induced nitrite release in rat islets and purified rat beta cells, nitrite formation and iNOS gene promoter activity in insulinoma cells, and iNOS mRNA expression in rat islets. The thiol depletor diethyl maleate (DEM) and an inhibitor of glutathione reductase 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) reduced IL-1 beta-stimulated nitrite release in islets. We conclude that GSH regulates IL-1 beta-induced NO production in islets, purified beta cells and insulinoma cells by modulation of iNOS gene expression.",

keywords = "Animals, Antineoplastic Agents, Alkylating, Buthionine Sulfoximine, Carmustine, Cell Separation, Cells, Cultured, Dose-Response Relationship, Drug, Enzyme Inhibitors, Flow Cytometry, Gene Expression, Glutathione, Insulin, Insulinoma, Interleukin-1, Islets of Langerhans, Maleates, Nitric Oxide, Nitric Oxide Synthase, Nitric Oxide Synthase Type II, Nitrites, Oxidation-Reduction, RNA, Messenger, Rats, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Journal Article, Research Support, Non-U.S. Gov't",

author = "Nikulina, \{M A\} and Andersen, \{H U\} and Karlsen, \{A E\} and Darville, \{M I\} and Eizirik, \{Decio Laks\} and T Mandrup-Poulsen",

year = "2000",

month = sep,

doi = "10.1006/cyto.2000.0712",

language = "English",

volume = "12",

pages = "1391--4",

journal = "Cytokine",

issn = "1043-4666",

publisher = "Academic Press",

number = "9",

}

TY - JOUR

T1 - Glutathione depletion inhibits IL-1 beta-stimulated nitric oxide production by reducing inducible nitric oxide synthase gene expression

AU - Nikulina, M A

AU - Andersen, H U

AU - Karlsen, A E

AU - Darville, M I

AU - Eizirik, Decio Laks

AU - Mandrup-Poulsen, T

PY - 2000/9

Y1 - 2000/9

N2 - L-buthionine-S,R-sulfoximine (BSO), an inhibitor of GSH synthesis, decreased IL-1 beta-induced nitrite release in rat islets and purified rat beta cells, nitrite formation and iNOS gene promoter activity in insulinoma cells, and iNOS mRNA expression in rat islets. The thiol depletor diethyl maleate (DEM) and an inhibitor of glutathione reductase 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) reduced IL-1 beta-stimulated nitrite release in islets. We conclude that GSH regulates IL-1 beta-induced NO production in islets, purified beta cells and insulinoma cells by modulation of iNOS gene expression.

AB - L-buthionine-S,R-sulfoximine (BSO), an inhibitor of GSH synthesis, decreased IL-1 beta-induced nitrite release in rat islets and purified rat beta cells, nitrite formation and iNOS gene promoter activity in insulinoma cells, and iNOS mRNA expression in rat islets. The thiol depletor diethyl maleate (DEM) and an inhibitor of glutathione reductase 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) reduced IL-1 beta-stimulated nitrite release in islets. We conclude that GSH regulates IL-1 beta-induced NO production in islets, purified beta cells and insulinoma cells by modulation of iNOS gene expression.

KW - Animals

KW - Antineoplastic Agents, Alkylating

KW - Buthionine Sulfoximine

KW - Carmustine

KW - Cell Separation

KW - Cells, Cultured

KW - Dose-Response Relationship, Drug

KW - Enzyme Inhibitors

KW - Flow Cytometry

KW - Gene Expression

KW - Glutathione

KW - Insulin

KW - Insulinoma

KW - Interleukin-1

KW - Islets of Langerhans

KW - Maleates

KW - Nitric Oxide

KW - Nitric Oxide Synthase

KW - Nitric Oxide Synthase Type II

KW - Nitrites

KW - Oxidation-Reduction

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Time Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1006/cyto.2000.0712

DO - 10.1006/cyto.2000.0712

M3 - Journal article

C2 - 10976000

SN - 1043-4666

VL - 12

SP - 1391

EP - 1394

JO - Cytokine

JF - Cytokine

IS - 9

ER -

Glutathione depletion inhibits IL-1 beta-stimulated nitric oxide production by reducing inducible nitric oxide synthase gene expression

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater