Global genotype-phenotype correlations in Pseudomonas aeruginosa

Claudia Pommerenke, Mathias Müsken, Tanja Becker, Andreas Dötsch, Frank Klawonn, Susanne Häussler

Abstract

Once the genome sequence of an organism is obtained, attention turns from identifying genes to understanding their function, their organization and control of metabolic pathways and networks that determine its physiology. Recent technical advances in acquiring genome-wide data have led to substantial progress in identifying gene functions. However, we still do not know the function of a large number of genes and, even when a gene product has been assigned to a functional class, we cannot normally predict its contribution to the phenotypic behaviour of the cell or organism--the phenome. In this study, we assessed bacterial growth parameters of 4030 non-redundant PA14 transposon mutants in the pathogenic bacterium Pseudomonas aeruginosa. The genome-wide simultaneous analysis of 119 distinct growth-related phenotypes uncovered a comprehensive phenome and provided evidence that most genotypes are not phenotypically isolated but rather define specific complex phenotypic clusters of genotypes. Since phenotypic overlap was demonstrated to reflect the relatedness of genotypes on a global scale, knowledge of an organism's phenome might significantly contribute to the advancement of functional genomics.

OriginalsprogEngelsk
TidsskriftP L o S Pathogens (Online)
Vol/bind6
Udgave nummer8
Sider (fra-til)e1001074
ISSN1553-7374
DOI
StatusUdgivet - 26 aug. 2010
Udgivet eksterntJa

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