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Genome-wide-analyses of Listeria monocytogenes from food-processing plants reveals clonal diversity and dates the emergence of persisting sequence types

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@article{d411087c33cf42d79c460c6f2376ed18,
title = "Genome-wide-analyses of Listeria monocytogenes from food-processing plants reveals clonal diversity and dates the emergence of persisting sequence types",
abstract = "Whole genome sequencing is increasing used in epidemiology, e.g. for tracing outbreaks of food-borne diseases. This requires in-depth understanding of pathogen emergence, persistence, and genomic diversity along the food production chain including in food processing plants. We sequenced the genomes of 80 isolates of Listeria monocytogenes sampled from Danish food processing plants over a time-period of 20 years, and analyzed the sequences together with 10 public available reference genomes to advance our understanding of inter- and intra-plant genomic diversity of L. monocytogenes. Except for three persisting sequence types (ST) based on Multi Locus Sequence Typing (MLST) being ST7, ST8 and ST121, long-term persistence of clonal groups was limited, and new clones were introduced continuously, potentially from raw materials. No particular gene could be linked to the persistence phenotype. Using time-based phylogenetic analyses of the persistent STs, we estimate the L. monocytogenes evolutionary rate to be 0.18-0.35 SNPs/year, suggesting that the persistent STs emerged approximately 100 years ago, which correlates with the onset of industrialization and globalization of the food market. This article is protected by copyright. All rights reserved.",
keywords = "Journal Article",
author = "Knudsen, {Gitte M} and Nielsen, {Jesper Boye} and Marvig, {Rasmus L} and Yin Ng and Peder Worning and Henrik Westh and Lone Gram",
note = "{\circledC} 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.",
year = "2017",
month = "8",
day = "1",
doi = "10.1111/1758-2229.12552",
language = "English",
volume = "9",
pages = "428--440",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Genome-wide-analyses of Listeria monocytogenes from food-processing plants reveals clonal diversity and dates the emergence of persisting sequence types

AU - Knudsen, Gitte M

AU - Nielsen, Jesper Boye

AU - Marvig, Rasmus L

AU - Ng, Yin

AU - Worning, Peder

AU - Westh, Henrik

AU - Gram, Lone

N1 - © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Whole genome sequencing is increasing used in epidemiology, e.g. for tracing outbreaks of food-borne diseases. This requires in-depth understanding of pathogen emergence, persistence, and genomic diversity along the food production chain including in food processing plants. We sequenced the genomes of 80 isolates of Listeria monocytogenes sampled from Danish food processing plants over a time-period of 20 years, and analyzed the sequences together with 10 public available reference genomes to advance our understanding of inter- and intra-plant genomic diversity of L. monocytogenes. Except for three persisting sequence types (ST) based on Multi Locus Sequence Typing (MLST) being ST7, ST8 and ST121, long-term persistence of clonal groups was limited, and new clones were introduced continuously, potentially from raw materials. No particular gene could be linked to the persistence phenotype. Using time-based phylogenetic analyses of the persistent STs, we estimate the L. monocytogenes evolutionary rate to be 0.18-0.35 SNPs/year, suggesting that the persistent STs emerged approximately 100 years ago, which correlates with the onset of industrialization and globalization of the food market. This article is protected by copyright. All rights reserved.

AB - Whole genome sequencing is increasing used in epidemiology, e.g. for tracing outbreaks of food-borne diseases. This requires in-depth understanding of pathogen emergence, persistence, and genomic diversity along the food production chain including in food processing plants. We sequenced the genomes of 80 isolates of Listeria monocytogenes sampled from Danish food processing plants over a time-period of 20 years, and analyzed the sequences together with 10 public available reference genomes to advance our understanding of inter- and intra-plant genomic diversity of L. monocytogenes. Except for three persisting sequence types (ST) based on Multi Locus Sequence Typing (MLST) being ST7, ST8 and ST121, long-term persistence of clonal groups was limited, and new clones were introduced continuously, potentially from raw materials. No particular gene could be linked to the persistence phenotype. Using time-based phylogenetic analyses of the persistent STs, we estimate the L. monocytogenes evolutionary rate to be 0.18-0.35 SNPs/year, suggesting that the persistent STs emerged approximately 100 years ago, which correlates with the onset of industrialization and globalization of the food market. This article is protected by copyright. All rights reserved.

KW - Journal Article

U2 - 10.1111/1758-2229.12552

DO - 10.1111/1758-2229.12552

M3 - Journal article

VL - 9

SP - 428

EP - 440

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 4

ER -

ID: 50563646