Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung

Adrian Kordes, Matthias Preusse, Sven D Willger, Peter Braubach, Danny Jonigk, Axel Haverich, Gregor Warnecke, Susanne Häussler

Abstract

Previous studies have demonstrated substantial genetic diversification of Pseudomonas aeruginosa across sub-compartments in cystic fibrosis (CF) lungs. Here, we isolate P. aeruginosa from five different sampling areas in the upper and lower airways of an explanted CF lung, analyze ex vivo transcriptional profiles by RNA-seq, and use colony re-sequencing and deep population sequencing to determine the genetic diversity within and across the various sub-compartments. We find that, despite genetic variation, the ex vivo transcriptional profiles of P. aeruginosa populations inhabiting different regions of the CF lung are similar. Although we cannot estimate the extent to which the transcriptional response recorded here actually reflects the in vivo transcriptomes, our results indicate that there may be a common in vivo transcriptional profile in the CF lung environment.

OriginalsprogEngelsk
TidsskriftNature Communications
Vol/bind10
Udgave nummer1
Sider (fra-til)3397
ISSN2041-1722
DOI
StatusUdgivet - 30 jul. 2019
Udgivet eksterntJa

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