TY - JOUR
T1 - Genetic profiles of gastroesophageal cancer: combined analysis using expression array and tiling array--comparative genomic hybridization
AU - Isinger-Ekstrand, Anna
AU - Johansson, Jan
AU - Ohlsson, Mattias
AU - Francis, Princy
AU - Staaf, Johan
AU - Jönsson, Mats
AU - Borg, Ake
AU - Nilbert, Mef
N1 - Copyright (c) 2010 Elsevier Inc. All rights reserved.
PY - 2010
Y1 - 2010
N2 - We aimed to characterize the genomic profiles of adenocarcinomas in the gastroesophageal junction in relation to cancers in the esophagus and the stomach. Profiles of gains/losses as well as gene expression profiles were obtained from 27 gastroesophageal adenocarcinomas by means of 32k high-resolution array-based comparative genomic hybridization and 27k oligo gene expression arrays, and putative target genes were validated in an extended series. Adenocarcinomas in the distal esophagus and the gastroesophageal junction showed strong similarities with the most common gains at 20q13, 8q24, 1q21-23, 5p15, 13q34, and 12q13, whereas different profiles with gains at 5p15, 7p22, 2q35, and 13q34 characterized gastric cancers. CDK6 and EGFR were identified as putative target genes in cancers of the esophagus and the gastroesophageal junction, with upregulation in one quarter of the tumors. Gains/losses and gene expression profiles show strong similarity between cancers in the distal esophagus and the gastroesophageal junction with frequent upregulation of CDK6 and EGFR, whereas gastric cancer displays distinct genetic changes. These data suggest that molecular diagnostics and targeted therapies can be applied to adenocarcinomas of the distal esophagus and gastroesophageal junction alike.
AB - We aimed to characterize the genomic profiles of adenocarcinomas in the gastroesophageal junction in relation to cancers in the esophagus and the stomach. Profiles of gains/losses as well as gene expression profiles were obtained from 27 gastroesophageal adenocarcinomas by means of 32k high-resolution array-based comparative genomic hybridization and 27k oligo gene expression arrays, and putative target genes were validated in an extended series. Adenocarcinomas in the distal esophagus and the gastroesophageal junction showed strong similarities with the most common gains at 20q13, 8q24, 1q21-23, 5p15, 13q34, and 12q13, whereas different profiles with gains at 5p15, 7p22, 2q35, and 13q34 characterized gastric cancers. CDK6 and EGFR were identified as putative target genes in cancers of the esophagus and the gastroesophageal junction, with upregulation in one quarter of the tumors. Gains/losses and gene expression profiles show strong similarity between cancers in the distal esophagus and the gastroesophageal junction with frequent upregulation of CDK6 and EGFR, whereas gastric cancer displays distinct genetic changes. These data suggest that molecular diagnostics and targeted therapies can be applied to adenocarcinomas of the distal esophagus and gastroesophageal junction alike.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Comparative Genomic Hybridization
KW - Cyclin-Dependent Kinase 6
KW - Esophageal Neoplasms
KW - Esophagogastric Junction
KW - Female
KW - Gene Dosage
KW - Gene Expression Profiling
KW - Humans
KW - Male
KW - Middle Aged
KW - Oligonucleotide Array Sequence Analysis
KW - Receptor, Epidermal Growth Factor
KW - Stomach Neoplasms
U2 - 10.1016/j.cancergencyto.2010.03.013
DO - 10.1016/j.cancergencyto.2010.03.013
M3 - Journal article
C2 - 20620594
SN - 1873-4456
SN - 2210-7770
VL - 200
SP - 120
EP - 126
JO - Cancer genetics and cytogenetics
JF - Cancer genetics and cytogenetics
IS - 2
ER -