Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1

Alisa Tubsuwan; Carlota Pires; Mikkel A Rasmussen; Benjamin Schmid; Jørgen E Nielsen; Lena E Hjermind; Vanessa Hall; Troels T Nielsen; Gunhild Waldemar; Poul Hyttel; Christian Clausen; Narisorn Kitiyanant; Kristine K Freude; Bjørn Holst

doi:10.1016/j.scr.2015.12.015

Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1

Alisa Tubsuwan, Carlota Pires, Mikkel A Rasmussen, Benjamin Schmid, Jørgen E Nielsen, Lena E Hjermind, Vanessa Hall, Troels T Nielsen, Gunhild Waldemar, Poul Hyttel, Christian Clausen, Narisorn Kitiyanant, Kristine K Freude, Bjørn Holst

Afdeling for Hjerne- og Nervesygdomme

19 Citationer (Scopus)

Abstract

Induced pluripotent stem cells (iPSCs) were generated from skin fibroblasts isolated from a 58-year old male with a L150P mutation in the presenilin 1 (PSEN-1) gene, which is responsible for the majority of familial cases of Alzheimer's disease (AD). The iPSCs were established by co-electroporation with episomal plasmids containing hOCT4, hSOX2, hL-MYC, hKLF4, hNANOG, hLIN28, and short hairpin RNA against TP53. The iPSCs contained the specific heterozygous mutation c.449C>T, had normal karyotype, expressed the expected pluripotency genes and displayed in vitro differentiation potential to the three germ layers. The iPSCs may be useful for studying familial AD pathology in vitro.

Originalsprog	Engelsk
Tidsskrift	Stem Cell Research
Vol/bind	16
Udgave nummer	1
Sider (fra-til)	110-2
Antal sider	3
ISSN	1873-5061
DOI	https://doi.org/10.1016/j.scr.2015.12.015
Status	Udgivet - jan. 2016

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10.1016/j.scr.2015.12.015

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Tubsuwan, A., Pires, C., Rasmussen, M. A., Schmid, B., Nielsen, J. E., Hjermind, L. E., Hall, V., Nielsen, T. T., Waldemar, G., Hyttel, P., Clausen, C., Kitiyanant, N., Freude, K. K., & Holst, B. (2016). Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1. Stem Cell Research, 16(1), 110-2. https://doi.org/10.1016/j.scr.2015.12.015

Tubsuwan, A, Pires, C, Rasmussen, MA, Schmid, B, Nielsen, JE, Hjermind, LE, Hall, V, Nielsen, TT , Waldemar, G, Hyttel, P, Clausen, C, Kitiyanant, N, Freude, KK & Holst, B 2016, 'Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1', Stem Cell Research, bind 16, nr. 1, s. 110-2. https://doi.org/10.1016/j.scr.2015.12.015

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title = "Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1",

abstract = "Induced pluripotent stem cells (iPSCs) were generated from skin fibroblasts isolated from a 58-year old male with a L150P mutation in the presenilin 1 (PSEN-1) gene, which is responsible for the majority of familial cases of Alzheimer's disease (AD). The iPSCs were established by co-electroporation with episomal plasmids containing hOCT4, hSOX2, hL-MYC, hKLF4, hNANOG, hLIN28, and short hairpin RNA against TP53. The iPSCs contained the specific heterozygous mutation c.449C>T, had normal karyotype, expressed the expected pluripotency genes and displayed in vitro differentiation potential to the three germ layers. The iPSCs may be useful for studying familial AD pathology in vitro.",

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author = "Alisa Tubsuwan and Carlota Pires and Rasmussen, {Mikkel A} and Benjamin Schmid and Nielsen, {J{\o}rgen E} and Hjermind, {Lena E} and Vanessa Hall and Nielsen, {Troels T} and Gunhild Waldemar and Poul Hyttel and Christian Clausen and Narisorn Kitiyanant and Freude, {Kristine K} and Bj{\o}rn Holst",

year = "2016",

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doi = "10.1016/j.scr.2015.12.015",

language = "English",

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T1 - Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1

AU - Tubsuwan, Alisa

AU - Pires, Carlota

AU - Rasmussen, Mikkel A

AU - Schmid, Benjamin

AU - Nielsen, Jørgen E

AU - Hjermind, Lena E

AU - Hall, Vanessa

AU - Nielsen, Troels T

AU - Waldemar, Gunhild

AU - Hyttel, Poul

AU - Clausen, Christian

AU - Kitiyanant, Narisorn

AU - Freude, Kristine K

AU - Holst, Bjørn

PY - 2016/1

Y1 - 2016/1

N2 - Induced pluripotent stem cells (iPSCs) were generated from skin fibroblasts isolated from a 58-year old male with a L150P mutation in the presenilin 1 (PSEN-1) gene, which is responsible for the majority of familial cases of Alzheimer's disease (AD). The iPSCs were established by co-electroporation with episomal plasmids containing hOCT4, hSOX2, hL-MYC, hKLF4, hNANOG, hLIN28, and short hairpin RNA against TP53. The iPSCs contained the specific heterozygous mutation c.449C>T, had normal karyotype, expressed the expected pluripotency genes and displayed in vitro differentiation potential to the three germ layers. The iPSCs may be useful for studying familial AD pathology in vitro.

AB - Induced pluripotent stem cells (iPSCs) were generated from skin fibroblasts isolated from a 58-year old male with a L150P mutation in the presenilin 1 (PSEN-1) gene, which is responsible for the majority of familial cases of Alzheimer's disease (AD). The iPSCs were established by co-electroporation with episomal plasmids containing hOCT4, hSOX2, hL-MYC, hKLF4, hNANOG, hLIN28, and short hairpin RNA against TP53. The iPSCs contained the specific heterozygous mutation c.449C>T, had normal karyotype, expressed the expected pluripotency genes and displayed in vitro differentiation potential to the three germ layers. The iPSCs may be useful for studying familial AD pathology in vitro.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.scr.2015.12.015

DO - 10.1016/j.scr.2015.12.015

M3 - Journal article

C2 - 27345792

SN - 1873-5061

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SP - 110

EP - 112

JO - Stem Cell Research

JF - Stem Cell Research

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ER -

Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1

Abstract

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