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Gene editing in the context of an increasingly complex genome

Publikation: Bidrag til tidsskriftReviewForskningpeer review

Harvard

Blighe, K, DeDionisio, L, Christie, KA, Chawes, B, Shareef, S, Kakouli-Duarte, T, Chao-Shern, C, Harding, V, Kelly, RS, Castellano, L, Stebbing, J, Lasky-Su, JA, Nesbit, MA & Moore, CBT 2018, 'Gene editing in the context of an increasingly complex genome' BMC Genomics, bind 19, nr. 1, s. 595. https://doi.org/10.1186/s12864-018-4963-8

APA

Blighe, K., DeDionisio, L., Christie, K. A., Chawes, B., Shareef, S., Kakouli-Duarte, T., ... Moore, C. B. T. (2018). Gene editing in the context of an increasingly complex genome. BMC Genomics, 19(1), 595. https://doi.org/10.1186/s12864-018-4963-8

CBE

Blighe K, DeDionisio L, Christie KA, Chawes B, Shareef S, Kakouli-Duarte T, Chao-Shern C, Harding V, Kelly RS, Castellano L, Stebbing J, Lasky-Su JA, Nesbit MA, Moore CBT. 2018. Gene editing in the context of an increasingly complex genome. BMC Genomics. 19(1):595. https://doi.org/10.1186/s12864-018-4963-8

MLA

Vancouver

Blighe K, DeDionisio L, Christie KA, Chawes B, Shareef S, Kakouli-Duarte T o.a. Gene editing in the context of an increasingly complex genome. BMC Genomics. 2018 aug 8;19(1):595. https://doi.org/10.1186/s12864-018-4963-8

Author

Blighe, K ; DeDionisio, L ; Christie, K A ; Chawes, B ; Shareef, S ; Kakouli-Duarte, T ; Chao-Shern, C ; Harding, V ; Kelly, R S ; Castellano, L ; Stebbing, J ; Lasky-Su, J A ; Nesbit, M A ; Moore, C B T. / Gene editing in the context of an increasingly complex genome. I: BMC Genomics. 2018 ; Bind 19, Nr. 1. s. 595.

Bibtex

@article{7d22f1cfa42a4bfa8f4ac2adcd1a5ec8,
title = "Gene editing in the context of an increasingly complex genome",
abstract = "The reporting of the first draft of the human genome in 2000 brought with it much hope for the future in what was felt as a paradigm shift toward improved health outcomes. Indeed, we have now mapped the majority of variation across human populations with landmark projects such as 1000 Genomes; in cancer, we have catalogued mutations across the primary carcinomas; whilst, for other diseases, we have identified the genetic variants with strongest association. Despite this, we are still awaiting the genetic revolution in healthcare to materialise and translate itself into the health benefits for which we had hoped. A major problem we face relates to our underestimation of the complexity of the genome, and that of biological mechanisms, generally. Fixation on DNA sequence alone and a 'rigid' mode of thinking about the genome has meant that the folding and structure of the DNA molecule -and how these relate to regulation- have been underappreciated. Projects like ENCODE have additionally taught us that regulation at the level of RNA is just as important as that at the spatiotemporal level of chromatin.In this review, we chart the course of the major advances in the biomedical sciences in the era pre- and post the release of the first draft sequence of the human genome, taking a focus on technology and how its development has influenced these. We additionally focus on gene editing via CRISPR/Cas9 as a key technique, in particular its use in the context of complex biological mechanisms. Our aim is to shift the mode of thinking about the genome to that which encompasses a greater appreciation of the folding of the DNA molecule, DNA- RNA/protein interactions, and how these regulate expression and elaborate disease mechanisms.Through the composition of our work, we recognise that technological improvement is conducive to a greater understanding of biological processes and life within the cell. We believe we now have the technology at our disposal that permits a better understanding of disease mechanisms, achievable through integrative data analyses. Finally, only with greater understanding of disease mechanisms can techniques such as gene editing be faithfully conducted.",
keywords = "Gene Editing/methods, Genetic Engineering, Genetic Variation, Genome, Human, Humans, RNA, Guide/genetics",
author = "K Blighe and L DeDionisio and Christie, {K A} and B Chawes and S Shareef and T Kakouli-Duarte and C Chao-Shern and V Harding and Kelly, {R S} and L Castellano and J Stebbing and Lasky-Su, {J A} and Nesbit, {M A} and Moore, {C B T}",
year = "2018",
month = "8",
day = "8",
doi = "10.1186/s12864-018-4963-8",
language = "English",
volume = "19",
pages = "595",
journal = "BMC Genomics",
issn = "1471-2164",
publisher = "BioMed Central Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Gene editing in the context of an increasingly complex genome

AU - Blighe, K

AU - DeDionisio, L

AU - Christie, K A

AU - Chawes, B

AU - Shareef, S

AU - Kakouli-Duarte, T

AU - Chao-Shern, C

AU - Harding, V

AU - Kelly, R S

AU - Castellano, L

AU - Stebbing, J

AU - Lasky-Su, J A

AU - Nesbit, M A

AU - Moore, C B T

PY - 2018/8/8

Y1 - 2018/8/8

N2 - The reporting of the first draft of the human genome in 2000 brought with it much hope for the future in what was felt as a paradigm shift toward improved health outcomes. Indeed, we have now mapped the majority of variation across human populations with landmark projects such as 1000 Genomes; in cancer, we have catalogued mutations across the primary carcinomas; whilst, for other diseases, we have identified the genetic variants with strongest association. Despite this, we are still awaiting the genetic revolution in healthcare to materialise and translate itself into the health benefits for which we had hoped. A major problem we face relates to our underestimation of the complexity of the genome, and that of biological mechanisms, generally. Fixation on DNA sequence alone and a 'rigid' mode of thinking about the genome has meant that the folding and structure of the DNA molecule -and how these relate to regulation- have been underappreciated. Projects like ENCODE have additionally taught us that regulation at the level of RNA is just as important as that at the spatiotemporal level of chromatin.In this review, we chart the course of the major advances in the biomedical sciences in the era pre- and post the release of the first draft sequence of the human genome, taking a focus on technology and how its development has influenced these. We additionally focus on gene editing via CRISPR/Cas9 as a key technique, in particular its use in the context of complex biological mechanisms. Our aim is to shift the mode of thinking about the genome to that which encompasses a greater appreciation of the folding of the DNA molecule, DNA- RNA/protein interactions, and how these regulate expression and elaborate disease mechanisms.Through the composition of our work, we recognise that technological improvement is conducive to a greater understanding of biological processes and life within the cell. We believe we now have the technology at our disposal that permits a better understanding of disease mechanisms, achievable through integrative data analyses. Finally, only with greater understanding of disease mechanisms can techniques such as gene editing be faithfully conducted.

AB - The reporting of the first draft of the human genome in 2000 brought with it much hope for the future in what was felt as a paradigm shift toward improved health outcomes. Indeed, we have now mapped the majority of variation across human populations with landmark projects such as 1000 Genomes; in cancer, we have catalogued mutations across the primary carcinomas; whilst, for other diseases, we have identified the genetic variants with strongest association. Despite this, we are still awaiting the genetic revolution in healthcare to materialise and translate itself into the health benefits for which we had hoped. A major problem we face relates to our underestimation of the complexity of the genome, and that of biological mechanisms, generally. Fixation on DNA sequence alone and a 'rigid' mode of thinking about the genome has meant that the folding and structure of the DNA molecule -and how these relate to regulation- have been underappreciated. Projects like ENCODE have additionally taught us that regulation at the level of RNA is just as important as that at the spatiotemporal level of chromatin.In this review, we chart the course of the major advances in the biomedical sciences in the era pre- and post the release of the first draft sequence of the human genome, taking a focus on technology and how its development has influenced these. We additionally focus on gene editing via CRISPR/Cas9 as a key technique, in particular its use in the context of complex biological mechanisms. Our aim is to shift the mode of thinking about the genome to that which encompasses a greater appreciation of the folding of the DNA molecule, DNA- RNA/protein interactions, and how these regulate expression and elaborate disease mechanisms.Through the composition of our work, we recognise that technological improvement is conducive to a greater understanding of biological processes and life within the cell. We believe we now have the technology at our disposal that permits a better understanding of disease mechanisms, achievable through integrative data analyses. Finally, only with greater understanding of disease mechanisms can techniques such as gene editing be faithfully conducted.

KW - Gene Editing/methods

KW - Genetic Engineering

KW - Genetic Variation

KW - Genome, Human

KW - Humans

KW - RNA, Guide/genetics

U2 - 10.1186/s12864-018-4963-8

DO - 10.1186/s12864-018-4963-8

M3 - Review

VL - 19

SP - 595

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

IS - 1

ER -

ID: 56447156