TY - JOUR
T1 - Gastroenteropancreatic tumours and prohormones
AU - Rehfeld, J F
AU - Bardram, L
AU - Hilsted, L
PY - 1996
Y1 - 1996
N2 - The structures and post-translational maturation of pancreatic and gastrointestinal prohormones are reviewed with emphasis on Danish contributions to today's knowledge. The review describes general, cell-specific, and tumour-specific prohormone-processing patterns. Since prohormone-processing in endocrine tumours is often attenuated, conventional assays that measure only the phenotypic endpoint of hormone gene expression (i.e. the bioactive hormone) do not quantitate tumour activity accurately. In contrast, measurements that include also prohormones and processing intermediates provide more accurate data on hormone synthesis in gastroenteropancreatic endocrine tumours. In order to comply with such demands we have developed a new analytical principle (processing-independent analysis (PIA)) which quantitates the entire translation product irrespective of the degree of processing. The significance of PIA in routine diagnostics awaits prospective evaluation. We hope that the present review illustrates how the tumour biology of endocrine cells in the pancreas and the gut has been an essential research area in Danish gastroenterology and endocrinology--one purpose being improvement of early diagnosis of endocrine tumours in the gut and the pancreas.
AB - The structures and post-translational maturation of pancreatic and gastrointestinal prohormones are reviewed with emphasis on Danish contributions to today's knowledge. The review describes general, cell-specific, and tumour-specific prohormone-processing patterns. Since prohormone-processing in endocrine tumours is often attenuated, conventional assays that measure only the phenotypic endpoint of hormone gene expression (i.e. the bioactive hormone) do not quantitate tumour activity accurately. In contrast, measurements that include also prohormones and processing intermediates provide more accurate data on hormone synthesis in gastroenteropancreatic endocrine tumours. In order to comply with such demands we have developed a new analytical principle (processing-independent analysis (PIA)) which quantitates the entire translation product irrespective of the degree of processing. The significance of PIA in routine diagnostics awaits prospective evaluation. We hope that the present review illustrates how the tumour biology of endocrine cells in the pancreas and the gut has been an essential research area in Danish gastroenterology and endocrinology--one purpose being improvement of early diagnosis of endocrine tumours in the gut and the pancreas.
KW - Denmark
KW - Gastrointestinal Hormones/metabolism
KW - Gastrointestinal Neoplasms/metabolism
KW - Gene Expression Regulation, Neoplastic
KW - Humans
KW - Neuroendocrine Tumors/metabolism
KW - Pancreatic Hormones/metabolism
KW - Pancreatic Neoplasms/metabolism
KW - Protein Processing, Post-Translational
U2 - 10.3109/00365529609094559
DO - 10.3109/00365529609094559
M3 - Review
C2 - 8726277
SN - 0085-5928
VL - 216
SP - 39
EP - 45
JO - Scandinavian Journal of Gastroenterology. Supplement
JF - Scandinavian Journal of Gastroenterology. Supplement
ER -