TY - JOUR
T1 - Future potential of in vitro maturation including fertility preservation
AU - Cadenas, Jesús
AU - la Cour Poulsen, Liv
AU - Mamsen, Linn Salto
AU - Andersen, Claus Yding
N1 - Copyright © 2023. Published by Elsevier Inc.
PY - 2023/4
Y1 - 2023/4
N2 - In several mammalian species, oocytes from small antral follicles after in vitro maturation (IVM) are successfully used for procreation. Humans are the exception, mainly because of limited access to immature oocytes and because oocyte maturation is uniquely regulated in women. With the introduction of cryopreservation of the ovarian cortex for fertility preservation, immature oocytes from small antral follicles in the medulla are now available for developing IVM on the basis of actual human studies. This review presents recent findings in favor of developing human IVM, including the oocyte diameter, follicle size from which the immature oocytes are collected, necessary level of follicle-stimulating hormone and luteinizing hormone to accelerate IVM, and secretion of factors from the cumulus-oocyte complex that affect the way oocyte maturation takes place. Furthermore, on the basis of studies in human granulosa cells and follicle fluid collected during the final maturation of follicles in vivo, a number of signal transduction pathways and hormone levels active during physiological conditions have been identified, providing new candidates and ways to improve the current IVM platform. Furthermore, it is suggested that the small droplet of culture medium in which IVM is performed mimics the hormonal milieu within a follicle created by the somatic cells and oocyte in vivo and may be used to advance oocyte nuclear and cytoplasmic maturation. Collectively, we envision that a continued research effort will develop a human IVM platform equally effective as for other mammalian species.
AB - In several mammalian species, oocytes from small antral follicles after in vitro maturation (IVM) are successfully used for procreation. Humans are the exception, mainly because of limited access to immature oocytes and because oocyte maturation is uniquely regulated in women. With the introduction of cryopreservation of the ovarian cortex for fertility preservation, immature oocytes from small antral follicles in the medulla are now available for developing IVM on the basis of actual human studies. This review presents recent findings in favor of developing human IVM, including the oocyte diameter, follicle size from which the immature oocytes are collected, necessary level of follicle-stimulating hormone and luteinizing hormone to accelerate IVM, and secretion of factors from the cumulus-oocyte complex that affect the way oocyte maturation takes place. Furthermore, on the basis of studies in human granulosa cells and follicle fluid collected during the final maturation of follicles in vivo, a number of signal transduction pathways and hormone levels active during physiological conditions have been identified, providing new candidates and ways to improve the current IVM platform. Furthermore, it is suggested that the small droplet of culture medium in which IVM is performed mimics the hormonal milieu within a follicle created by the somatic cells and oocyte in vivo and may be used to advance oocyte nuclear and cytoplasmic maturation. Collectively, we envision that a continued research effort will develop a human IVM platform equally effective as for other mammalian species.
KW - fertility preservation
KW - Human oocyte maturation
KW - IVM
KW - recreating follicle environment in vitro
KW - small antral follicles
KW - Ovarian Follicle
KW - Humans
KW - Mammals
KW - Fertility Preservation
KW - Oogenesis
KW - Animals
KW - Cryopreservation
KW - Female
KW - Oocytes/physiology
KW - In Vitro Oocyte Maturation Techniques
U2 - 10.1016/j.fertnstert.2023.01.027
DO - 10.1016/j.fertnstert.2023.01.027
M3 - Journal article
C2 - 36702341
SN - 0015-0282
VL - 119
SP - 550
EP - 559
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 4
ER -