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Udgivet

Full-Length Open Reading Frame Amplification of Hepatitis C Virus

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Analysis of Mass Cytometry Data

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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The purpose of this method is to amplify the full coding sequence of hepatitis C virus (HCV) by a single round reverse transcriptase-polymerase chain reaction (RT-PCR) approach. Our method relies on a highly robust and sensitive RNA extraction procedure and cutting-edge RT-PCR enzymes, all of which have been rigorously tested and optimized. This will not only allow for robust amplification of the entire open reading frame (ORF) of HCV for sequencing by Sanger or next-generation sequencing (NGS), but can also be used for cloning of the ORF of uncharacterized samples and for linkage analysis of mutations on individual genomes spanning the entire ORF. The method has been validated on a variety of samples, including sera from HCV patients and cell-culture supernatants.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology
Vol/bind1911
Sider (fra-til)85-91
Antal sider7
ISSN1064-3745
DOI
StatusUdgivet - 2019

ID: 56118500