Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Fructose malabsorption induces cholecystokinin expression in the ileum and cecum by changing microbiota composition and metabolism

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Early development of tendinopathy in humans: Sequence of pathological changes in structure and tissue turnover signaling

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Muscle-strain injury exudate favors acute tissue healing and prolonged connective tissue formation in humans

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Oral D/L-3-Hydroxybutyrate stimulates cholecystokinin and insulin secretion and slows gastric emptying in healthy males

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Bilio-enteric flow and plasma concentrations of bile acids after gastric bypass and sleeve gastrectomy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Increased oral sodium chloride intake in humans amplifies selectively postprandial GLP-1 but not GIP, CCK, and gastrin in plasma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Changes in the homeostatic appetite system after weight loss reflect a normalization toward a lower body weight

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Xufei Zhang
  • Alexandra Grosfeld
  • Edek Williams
  • Daniel Vasiliauskas
  • Sharon Barretto
  • Lorraine Smith
  • Mahendra Mariadassou
  • Catherine Philippe
  • Fabienne Devime
  • Chloé Melchior
  • Guillaume Gourcerol
  • Nathalie Dourmap
  • Nicolas Lapaque
  • Pierre Larraufie
  • Hervé M Blottière
  • Christine Herberden
  • Philippe Gerard
  • Jens F Rehfeld
  • Ronaldo P Ferraris
  • J Christopher Fritton
  • Sandrine Ellero-Simatos
  • Veronique Douard
Vis graf over relationer

Current fructose consumption levels often overwhelm the intestinal capacity to absorb fructose. We investigated the impact of fructose malabsorption on intestinal endocrine function and addressed the role of the microbiota in this process. To answer this question, a mouse model of moderate fructose malabsorption [ketohexokinase mutant (KHK)-/-] and wild-type (WT) littermate mice were used and received a 20%-fructose (KHK-F and WT-F) or 20%-glucose diet. Cholecystokinin (Cck) mRNA and protein expression in the ileum and cecum, as well as preproglucagon (Gcg) and neurotensin (Nts) mRNA expression in the cecum, increased in KHK-F mice. In KHK-F mice, triple-label immunohistochemistry showed major up-regulation of CCK in enteroendocrine cells (EECs) that were glucagon-like peptide-1 (GLP-1)+/Peptide YY (PYY-) in the ileum and colon and GLP-1-/PYY- in the cecum. The cecal microbiota composition was drastically modified in the KHK-F in association with an increase in glucose, propionate, succinate, and lactate concentrations. Antibiotic treatment abolished fructose malabsorption-dependent induction of cecal Cck mRNA expression and, in mouse GLUTag and human NCI-H716 cells, Cck mRNA expression levels increased in response to propionate, both suggesting a microbiota-dependent process. Fructose reaching the lower intestine can modify the composition and metabolism of the microbiota, thereby stimulating the production of CCK from the EECs possibly in response to propionate.-Zhang, X., Grosfeld, A., Williams, E., Vasiliauskas, D., Barretto, S., Smith, L., Mariadassou, M., Philippe, C., Devime, F., Melchior, C., Gourcerol, G., Dourmap, N., Lapaque, N., Larraufie, P., Blottière, H. M., Herberden, C., Gerard, P., Rehfeld, J. F., Ferraris, R. P., Fritton, J. C., Ellero-Simatos, S., Douard, V. Fructose malabsorption induces cholecystokinin expression in the ileum and cecum by changing microbiota composition and metabolism.

OriginalsprogEngelsk
TidsskriftFASEB Journal
Vol/bind33
Udgave nummer6
Sider (fra-til)7126-7142
Antal sider17
ISSN0892-6638
DOI
StatusUdgivet - jun. 2019

ID: 58077129