Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Follicular hormone dynamics during the midcycle surge of gonadotropins in women undergoing fertility treatment

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Effect of sphingosine-1-phosphate on activation of dormant follicles in murine and human ovarian tissue

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Education, education, education-now more than ever?

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Inhibin-B secretion and FSH isoform distribution may play an integral part of follicular selection in the natural menstrual cycle

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Reply: Methodological considerations in measuring different AMH splice forms using ELISA: validity of proAMH ELISA

    Publikation: Bidrag til tidsskriftKommentar/debatForskningpeer review

  1. Association between women's age and stage, morphology, and implantation of the competent blastocyst: a multicenter cohort study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Identification of a unique epigenetic profile in women with diminished ovarian reserve

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. N-acetylcysteine protects ovarian follicles from ischemia-reperfusion injury in xenotransplanted human ovarian tissue

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Changes in concentrations of intra-follicular hormones during ovulation are important for final oocyte maturation and endometrial priming to ensure reproductive success. As no human studies have investigated these changes in detail, our objective was to describe the dynamics of major follicular fluid (FF) hormones and transcription of steroidogenic enzymes and steroid receptors in human granulosa cells (GCs) during ovulation. We conducted a prospective cohort study at a public fertility clinic in 2016-2018. Fifty women undergoing ovarian stimulation for fertility treatment were included. From each woman, FF and GCs were collected by transvaginal ultrasound-guided follicle puncture of one follicle at two specific time points during ovulation, and the study covered a total of five time points: before ovulation induction (OI), 12, 17, 32 and 36 h after OI. Follicular fluid concentrations of oestradiol, progesterone, androstenedione, testosterone, 17-hydroxyprogesterone, anti-Mullerian hormone, inhibin A and inhibin B were measured using ELISA assays, and a statistical mixed model was used to analyse differences in hormone levels between time points. Gene expression of 33 steroidogenic enzymes and six hormone receptors in GCs across ovulation were assessed by microarray analysis, and selected genes were validated by quantitative reverse transcription PCR. We found that concentrations of oestradiol, testosterone, progesterone, AMH, inhibin A and inhibin B (P < 0.001) and gene expression of 12 steroidogenic enzymes and five receptors (false discovery rate < 0.0001) changed significantly during ovulation. Furthermore, we found parallel changes in plasma hormones. The substantial changes in follicular hormone production during ovulation highlight their importance for reproductive success.

OriginalsprogEngelsk
TidsskriftMolecular Human Reproduction
Vol/bind26
Udgave nummer4
Sider (fra-til)256-268
Antal sider13
ISSN1360-9947
DOI
StatusUdgivet - 15 apr. 2020

Bibliografisk note

© The Author(s) 2020. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.

ID: 61255721