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Region Hovedstaden - en del af Københavns Universitetshospital
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Flow cytometry analysis of T-cell subsets in cerebrospinal fluid of narcolepsy type 1 patients with long-lasting disease

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  1. CD8+ T cells from patients with narcolepsy and healthy controls recognize hypocretin neuron-specific antigens

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. In vivo clonal expansion and phenotypes of hypocretin-specific CD4+ T cells in narcolepsy patients and controls

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Altered surface expression of P2Y11 receptor with narcolepsy-associated mutations

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  4. Type 1 narcolepsy is not present in 29 HPV-vaccinated individuals with subjective sleep complaints

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  5. DNMT1 regulates expression of MHC class i in post-mitotic neurons

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Monica Moresco
  • Mariangela Lecciso
  • Darina Ocadlikova
  • Marco Filardi
  • Silvia Melzi
  • Birgitte Rahbek Kornum
  • Elena Antelmi
  • Fabio Pizza
  • Emmanuel Mignot
  • Antonio Curti
  • Giuseppe Plazzi
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Background: Type 1 narcolepsy (NT1) is a central hypersomnia linked to the destruction of hypocretin-producing neurons. A great body of genetic and epidemiological data points to likely autoimmune disease aetiology. Recent reports have characterized peripheral blood T-cell subsets in NT1, whereas data regarding the cerebrospinal fluid (CSF) immune cell composition are lacking. The current study aimed to characterize the T-cell and natural killer (NK) cell subsets in NT1 patients with long disease course. Methods: Immune cell subsets from CSF and peripheral blood mononuclear cell (PBMC) samples were analysed by flow cytometry in two age-balanced and sex-balanced groups of 14 NT1 patients versus 14 healthy controls. The frequency of CSF cell groups was compared with PBMCs. Non-parametric tests were used for statistical analyses. Results: The NT1 patients did not show significant differences of CSF immune cell subsets compared to controls, despite a trend towards higher CD4+ terminally differentiated effector memory T cells. T cells preferentially displayed a memory phenotype in the CSF compared to PBMCs. Furthermore, a reduced frequency of CD4+ terminally differentiated effector memory T cells and an increased frequency of NK CD56bright cells was observed in PBMCs from patients compared to controls. Finally, the ratio between CSF and peripheral CD4+ terminally differentiated effector memory T cells was two-fold increased in NT1 patients versus controls. Conclusions: Significant differences in PBMCs and in CSF/PBMC ratios of immune cell profile were found in NT1 patients compared to healthy controls. These differences might have arisen from the different HLA status, or be primary or secondary to hypocretin deficiency. Further functional studies in patients close to disease onset are required to understand NT1 pathophysiology.

OriginalsprogEngelsk
TidsskriftSleep Medicine
Vol/bind44
Sider (fra-til)53-60
Antal sider8
ISSN1389-9457
DOI
StatusUdgivet - 1 apr. 2018

ID: 55697233