Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Filaggrin Expression and Processing Deficiencies Impair Corneocyte Surface Texture and Stiffness in Mice

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Enhanced and Sustained Cutaneous Delivery of Vismodegib by Ablative Fractional Laser and Microemulsion Formulation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Melanin has a Small Inhibitory Effect on Cutaneous Vitamin D Synthesis: A Comparison of Extreme Phenotypes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Predictive Metagenomic Analysis Reveals a Role of Cutaneous Dysbiosis in the Development of Hidradenitis Suppurativa

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Pathogenic CD8+ Epidermis-Resident Memory T Cells Displace Dendritic Epidermal T Cells in Allergic Dermatitis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Overall Survival in Mycosis Fungoides: A Systematic Review and Meta-Analysis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. The stratum corneum transcriptome in atopic dermatitis can be assessed by tape stripping

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Normal insulin sensitivity, glucose tolerance, gut incretin and pancreatic hormone responses in adults with atopic dermatitis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Jacob P Thyssen
  • Ivone Jakasa
  • Christoph Riethmüller
  • Michael P Schön
  • Andrea Braun
  • Marek Haftek
  • Padraic G Fallon
  • Jacek Wróblewski
  • Hieronim Jakubowski
  • Leopold Eckhart
  • Wim Declercq
  • Sjors Koppes
  • Kristiane A Engebretsen
  • Charlotte Bonefeld
  • Alan D Irvine
  • Sokhna Keita-Alassane
  • Michel Simon
  • Hiroshi Kawasaki
  • Akiharu Kubo
  • Masayuki Amagai
  • Takeshi Matsui
  • Sanja Kezic
Vis graf over relationer

Abundant corneocyte surface protrusions, observed in patients with atopic dermatitis with filaggrin loss-of-function mutations, are inversely associated with levels of natural moisturizing factors (NMFs) in the stratum corneum. To dissect the etiological role of NMFs and filaggrin deficiency in surface texture alterations, we examined mouse models with genetic deficiencies in the synthesis or degradation of filaggrin monomers for NMFs, cell stiffness (elastic modulus) and corneocyte surface protrusion density (dermal texture index). Five neonatal and adult mouse models carrying inactivating mutations of SASPase (Sasp-/-), filaggrin (Flgft/ft and Flg-/-), filaggrin-hornerin (FlgHrnr-/-), and bleomycin hydrolase (Blmh-/-) were investigated. Sasp-/- and Flg-/- were on the hairless mouse background. Atomic force microscopy was used to determine elastic modulus and dermal texture index. Corneocytes of each neonatal as well as hairless adult knockout mouse exhibited an increased number of protrusions and decreased elastic modulus. In these mice, NMFs were reduced except for Sasp-/-. Dermal texture index was inversely correlated with NMFs and elastic modulus. Our findings demonstrate that any filaggrin-NMF axis deficiency can affect corneocyte mechanical properties in mice and likely in humans. Differences in NMFs and corneocyte surface texture between neonatal and adult as well as hairless and hairy mice emphasize the need for carefully selecting the most appropriate animal models for studies.

OriginalsprogEngelsk
TidsskriftThe Journal of investigative dermatology
Vol/bind140
Udgave nummer3
Sider (fra-til)615-623.e5
ISSN0022-202X
DOI
StatusUdgivet - mar. 2020

Bibliografisk note

Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

ID: 60481350