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Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Fecal sample preparation for untargeted and targeted metabolomics and a reproducibility study in the targeted platform

Publikation: KonferencebidragKonferenceabstrakt til konferenceForskningpeer review

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Vis graf over relationer
Sample preparation is an essential step to obtain high quality data in MS-based clinical metabolomics. For screening metabolites in heterogeneous semi-solid biological samples through multiple platforms, an ideal sample preparation requires a fast and reproducible method to maximize metabolite detection/quantification. Herein, we propose an efficient and robust method for preparing fecal samples for metabolomics analyses in different MS platforms. We describe a step-wise method that was accordingly derivatized for metabolomics analysis by GCGC-MS, untargeted lipidomics analysis by UPLC-qTof-MS, targeted metabolomics analyses by LC-ESI-MS/MS. Our method has been focused on reducing aliquoting and producing a common sample that covers a large variety of polar and non-polar metabolites of interest for clinical research. Here we present results from the targeted platform for a broad coverage of n=25 metabolites that represent liver-gut axis metabolism. Our platform test cohort consisted of 475 samples from patients with a history of harmful drinking and healthy controls.
OriginalsprogEngelsk
Publikationsdatookt. 2020
StatusUdgivet - okt. 2020
Begivenhed16th Annual Conference of the Metabolomics Society -
Varighed: 27 okt. 202029 okt. 2020
https://metabolomics.eventsair.com/metabolomics-2020-online/

Konference

Konference16th Annual Conference of the Metabolomics Society
Periode27/10/202029/10/2020
Internetadresse

Begivenhed

16th Annual Conference of the Metabolomics Society

27/10/202029/10/2020

Begivenhed: Konference

ID: 61503775