Feasibility of capillary liquid chromatography/microchip atmospheric pressure photoionization mass spectrometry in analyzing anabolic steroids in urine samples

Linda L. Ahonen, Markus Haapla, Ville Saarela, Sami Franssila, Tapio Kotiaho, Risto Kostiainen*

*Corresponding author af dette arbejde
18 Citationer (Scopus)

Abstract

We examined the feasibility of capillary liquid chromatography/microchip atmospheric pressure photoionization tandem mass spectrometry (capLC/μAPPI-MS/MS) for the analysis of anabolic steroids in human urine. The urine samples were pretreated by enzymatic hydrolysis (with β-glucuronidase from Helix pomatia), and the compounds were liquid-liquid extracted with diethyl ether. After separation the compounds were vaporized by microchip APPI, photoionized by a 10 eV krypton discharge lamp, and detected by selected reaction monitoring. The capLC/μAPPI-MS/MS method showed good sensitivity with detection limits at the level of 1.0 ng mL-1, good linearity with correlation coefficients between 0.9954 and 0.9990, and good repeatability with relative standard deviations below 10%. These results demonstrate that microchip APPI combined with capLC/MS/MS provides a new potential method for analyzing non-polar and neutral compounds in biological samples.

OriginalsprogEngelsk
TidsskriftRapid Communications in Mass Spectrometry
Vol/bind24
Udgave nummer7
Sider (fra-til)958-964
Antal sider7
ISSN0951-4198
DOI
StatusUdgivet - 1 jan. 2010
Udgivet eksterntJa

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