Abstract
We examined the feasibility of capillary liquid chromatography/microchip atmospheric pressure photoionization tandem mass spectrometry (capLC/μAPPI-MS/MS) for the analysis of anabolic steroids in human urine. The urine samples were pretreated by enzymatic hydrolysis (with β-glucuronidase from Helix pomatia), and the compounds were liquid-liquid extracted with diethyl ether. After separation the compounds were vaporized by microchip APPI, photoionized by a 10 eV krypton discharge lamp, and detected by selected reaction monitoring. The capLC/μAPPI-MS/MS method showed good sensitivity with detection limits at the level of 1.0 ng mL-1, good linearity with correlation coefficients between 0.9954 and 0.9990, and good repeatability with relative standard deviations below 10%. These results demonstrate that microchip APPI combined with capLC/MS/MS provides a new potential method for analyzing non-polar and neutral compounds in biological samples.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Rapid Communications in Mass Spectrometry |
Vol/bind | 24 |
Udgave nummer | 7 |
Sider (fra-til) | 958-964 |
Antal sider | 7 |
ISSN | 0951-4198 |
DOI | |
Status | Udgivet - 1 jan. 2010 |
Udgivet eksternt | Ja |