Ex vivo intestinal adhesion of Escherichia coli LF82 in Crohn's disease

Stina Rikke Jensen; Lisbeth Nielsen Fink; Ole Haagen Nielsen; Jørn Brynskov; Susanne Brix

doi:10.1016/j.micpath.2011.08.006

Ex vivo intestinal adhesion of Escherichia coli LF82 in Crohn's disease

Stina Rikke Jensen, Lisbeth Nielsen Fink, Ole Haagen Nielsen, Jørn Brynskov, Susanne Brix

Mave-, Tarm- og Leversygdomme

10 Citationer (Scopus)

Abstrakt

Adherent-invasive Escherichia coli (AIEC) are reported to inhabit the gut mucosa in Crohn's disease (CD), however, little is known about the importance of host factors for the interplay between AIEC and the human gut. To examine if differences in bacterial adhesion patterns are disease associated, the AIEC-prototype strain LF82 was evaluated for its ability to adhere to ileal and colonic biopsies from CD and healthy controls (HC). Moreover, the efficacy of the non-pathogenic E. coli Nissle 1917 (ECN) in averting LF82 adhesion to ileal mucosa was assessed. Similar numbers of LF82 adhered to biopsies from CD and HC. A significantly greater LF82 attachment to ileal versus colonic mucosa was found in HC (P

Originalsprog	Engelsk
Tidsskrift	Microbial Pathogenesis
Vol/bind	51
Udgave nummer	6
Sider (fra-til)	426-31
Antal sider	6
ISSN	0882-4010
DOI	https://doi.org/10.1016/j.micpath.2011.08.006
Status	Udgivet - 2011

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10.1016/j.micpath.2011.08.006

Citationsformater

@article{3e2b28fc1f894ea793bb2b06d508e820,

title = "Ex vivo intestinal adhesion of Escherichia coli LF82 in Crohn's disease",

abstract = "Adherent-invasive Escherichia coli (AIEC) are reported to inhabit the gut mucosa in Crohn's disease (CD), however, little is known about the importance of host factors for the interplay between AIEC and the human gut. To examine if differences in bacterial adhesion patterns are disease associated, the AIEC-prototype strain LF82 was evaluated for its ability to adhere to ileal and colonic biopsies from CD and healthy controls (HC). Moreover, the efficacy of the non-pathogenic E. coli Nissle 1917 (ECN) in averting LF82 adhesion to ileal mucosa was assessed. Similar numbers of LF82 adhered to biopsies from CD and HC. A significantly greater LF82 attachment to ileal versus colonic mucosa was found in HC (P ",

keywords = "Adolescent, Adult, Bacterial Adhesion, Bacterial Load, Biopsy, Colon, Crohn Disease, Escherichia coli, Female, Humans, Ileum, Intestinal Mucosa, Male, Middle Aged, Organ Culture Techniques, Young Adult",

author = "Jensen, {Stina Rikke} and Fink, {Lisbeth Nielsen} and Nielsen, {Ole Haagen} and J{\o}rn Brynskov and Susanne Brix",

year = "2011",

doi = "10.1016/j.micpath.2011.08.006",

language = "English",

volume = "51",

pages = "426--31",

journal = "Microbial Pathogenesis",

issn = "0882-4010",

publisher = "Academic Press",

number = "6",

}

TY - JOUR

T1 - Ex vivo intestinal adhesion of Escherichia coli LF82 in Crohn's disease

AU - Jensen, Stina Rikke

AU - Fink, Lisbeth Nielsen

AU - Nielsen, Ole Haagen

AU - Brynskov, Jørn

AU - Brix, Susanne

PY - 2011

Y1 - 2011

N2 - Adherent-invasive Escherichia coli (AIEC) are reported to inhabit the gut mucosa in Crohn's disease (CD), however, little is known about the importance of host factors for the interplay between AIEC and the human gut. To examine if differences in bacterial adhesion patterns are disease associated, the AIEC-prototype strain LF82 was evaluated for its ability to adhere to ileal and colonic biopsies from CD and healthy controls (HC). Moreover, the efficacy of the non-pathogenic E. coli Nissle 1917 (ECN) in averting LF82 adhesion to ileal mucosa was assessed. Similar numbers of LF82 adhered to biopsies from CD and HC. A significantly greater LF82 attachment to ileal versus colonic mucosa was found in HC (P

AB - Adherent-invasive Escherichia coli (AIEC) are reported to inhabit the gut mucosa in Crohn's disease (CD), however, little is known about the importance of host factors for the interplay between AIEC and the human gut. To examine if differences in bacterial adhesion patterns are disease associated, the AIEC-prototype strain LF82 was evaluated for its ability to adhere to ileal and colonic biopsies from CD and healthy controls (HC). Moreover, the efficacy of the non-pathogenic E. coli Nissle 1917 (ECN) in averting LF82 adhesion to ileal mucosa was assessed. Similar numbers of LF82 adhered to biopsies from CD and HC. A significantly greater LF82 attachment to ileal versus colonic mucosa was found in HC (P

KW - Adolescent

KW - Adult

KW - Bacterial Adhesion

KW - Bacterial Load

KW - Biopsy

KW - Colon

KW - Crohn Disease

KW - Escherichia coli

KW - Female

KW - Humans

KW - Ileum

KW - Intestinal Mucosa

KW - Male

KW - Middle Aged

KW - Organ Culture Techniques

KW - Young Adult

U2 - 10.1016/j.micpath.2011.08.006

DO - 10.1016/j.micpath.2011.08.006

M3 - Journal article

C2 - 21911052

VL - 51

SP - 426

EP - 431

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

SN - 0882-4010

IS - 6

ER -

Ex vivo intestinal adhesion of Escherichia coli LF82 in Crohn's disease

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