Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands

C Kragelund; C Hansen; L A Torpet; B Nauntofte; K Brøsen; A M L Pedersen; C Buchwald; M H Therkildsen; J Reibel

doi:10.1111/j.1601-0825.2007.01415.x

Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands

C Kragelund, C Hansen, L A Torpet, B Nauntofte, K Brøsen, A M L Pedersen, C Buchwald, M H Therkildsen, J Reibel

Abstract

OBJECTIVE: The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands.

METHODS: Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA.

RESULTS: CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent.

CONCLUSION: The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva.

Originalsprog	Engelsk
Tidsskrift	Oral Diseases
Vol/bind	14
Udgave nummer	6
Sider (fra-til)	533-40
Antal sider	8
ISSN	1354-523X
DOI	https://doi.org/10.1111/j.1601-0825.2007.01415.x
Status	Udgivet - sep. 2008
Udgivet eksternt	Ja

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10.1111/j.1601-0825.2007.01415.x

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title = "Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands",

abstract = "OBJECTIVE: The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands.METHODS: Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA.RESULTS: CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent.CONCLUSION: The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva.",

keywords = "Adult, Aged, Aged, 80 and over, Alcohol Drinking/metabolism, Cytochrome P-450 CYP1A2/analysis, Cytochrome P-450 CYP3A/analysis, Female, Gene Expression Regulation, Enzymologic, Humans, Immunohistochemistry, In Situ Hybridization, Male, Middle Aged, Mucous Membrane/enzymology, Parotid Gland/enzymology, Salivary Ducts/enzymology, Salivary Glands/enzymology, Salivary Glands, Minor/enzymology, Salivary Proteins and Peptides/analysis, Serous Membrane/enzymology, Smoking/metabolism, Submandibular Gland/enzymology, Xenobiotics/metabolism, Young Adult",

author = "C Kragelund and C Hansen and Torpet, {L A} and B Nauntofte and K Br{\o}sen and Pedersen, {A M L} and C Buchwald and Therkildsen, {M H} and J Reibel",

year = "2008",

month = sep,

doi = "10.1111/j.1601-0825.2007.01415.x",

language = "English",

volume = "14",

pages = "533--40",

journal = "Oral Diseases",

issn = "1354-523X",

publisher = "Wiley-Blackwell Munksgaard",

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}

TY - JOUR

T1 - Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands

AU - Kragelund, C

AU - Hansen, C

AU - Torpet, L A

AU - Nauntofte, B

AU - Brøsen, K

AU - Pedersen, A M L

AU - Buchwald, C

AU - Therkildsen, M H

AU - Reibel, J

PY - 2008/9

Y1 - 2008/9

N2 - OBJECTIVE: The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands.METHODS: Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA.RESULTS: CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent.CONCLUSION: The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva.

AB - OBJECTIVE: The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands.METHODS: Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA.RESULTS: CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent.CONCLUSION: The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Alcohol Drinking/metabolism

KW - Cytochrome P-450 CYP1A2/analysis

KW - Cytochrome P-450 CYP3A/analysis

KW - Female

KW - Gene Expression Regulation, Enzymologic

KW - Humans

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Male

KW - Middle Aged

KW - Mucous Membrane/enzymology

KW - Parotid Gland/enzymology

KW - Salivary Ducts/enzymology

KW - Salivary Glands/enzymology

KW - Salivary Glands, Minor/enzymology

KW - Salivary Proteins and Peptides/analysis

KW - Serous Membrane/enzymology

KW - Smoking/metabolism

KW - Submandibular Gland/enzymology

KW - Xenobiotics/metabolism

KW - Young Adult

U2 - 10.1111/j.1601-0825.2007.01415.x

DO - 10.1111/j.1601-0825.2007.01415.x

M3 - Journal article

C2 - 18266837

SN - 1354-523X

VL - 14

SP - 533

EP - 540

JO - Oral Diseases

JF - Oral Diseases

IS - 6

ER -

Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands

Abstract

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