Expression of two drug-metabolizing cytochrome P450-enzymes in human salivary glands

C Kragelund, C Hansen, L A Torpet, B Nauntofte, K Brøsen, A M L Pedersen, C Buchwald, M H Therkildsen, J Reibel


OBJECTIVE: The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics and / or their metabolites in the saliva may affect the oral mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 and CYP3A4 are expressed in salivary glands.

METHODS: Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) and labial (10) salivary glands were examined immunohistochemically and by in situ hybridization for expression of CYP1A2 and CYP3A4 protein and mRNA.

RESULTS: CYP1A2 and CYP3A4 protein and mRNA were detected in ductal and seromucous / serous acinar cells in all gland types although to a varying degree and intensity. Mucous acinar cells were positive to a lesser extent.

CONCLUSION: The results indicate a xenobiotic metabolizing capability of salivary glands. This may have implications for development of oral mucosal disease as a result of mucosal exposure to metabolites originating from internal sources (blood) as well as from saliva.

TidsskriftOral Diseases
Udgave nummer6
Sider (fra-til)533-40
Antal sider8
StatusUdgivet - sep. 2008
Udgivet eksterntJa


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