Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Expression of the Insulin-like Growth Factor System in First- and Second-Trimester Human Embryonic and Fetal Gonads

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Expression of cholecystokinin and its receptors in the intestinal tract of type 2 diabetes patients and healthy controls

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Elevated apolipoprotein A1 and HDL cholesterol associated with age-related macular degeneration: 2 population cohorts

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Prenatal Exposure to Butyl Paraben Is Associated With Fat Percentage in 7-Year-Old Boys

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Serum insulin-like factor 3 levels are reduced in former androgen users suggesting impaired Leydig cell capacity

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Treatment of Hyperthyroidism Reduces Systemic Oxidative Stress, as Measured by Markers of RNA and DNA Damage

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. N-acetylcysteine protects ovarian follicles from ischemia-reperfusion injury in xenotransplanted human ovarian tissue

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Umbilical cord blood-derived platelet-rich plasma: a clinically acceptable substitute for fetal bovine serum?

    Publikation: Bidrag til tidsskriftLederForskningpeer review

  4. Parental Acceptance Rate of Testicular Tissue Cryopreservation in Danish Boys with Cryptorchidism

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Biological and Clinical Rationale for Androgen Priming in Ovarian Stimulation

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

Vis graf over relationer

CONTEXT: Insulin-like growth factor (IGF) signaling is crucial for sex differentiation and development of Leydig and Sertoli cells in fetal mice testes. No such information is available for human embryonic and fetal testes and ovaries.

OBJECTIVE: To investigate presence and activity of the IGF signaling system during human embryonic and fetal ovarian and testicular development.

DESIGN: Human embryonic and fetal gonads were obtained following legal terminations of pregnancies. Gene expression was assessed by microarray and qPCR transcript analyses. Proteins of the IGF system components were detected with immunohistochemistry and immunofluorescence analyses. Specimens were included from 2010 to 2017.

SETTING: University Hospital.

PATIENTS/PARTICIPANTS: Ovaries and testes from a total of 124 human embryos and fetuses aged 5 to 17 postconception weeks were obtained from healthy women aged 16 to 47 years resident in Denmark or Scotland.

MAIN OUTCOME MEASURES: Gene expression analysis using microarray was performed in 46 specimens and qPCR analysis in 56 specimens, both sexes included. Protein analysis included 22 specimens (11 ovaries, 11 testes).

RESULTS: IGF system members were detected in embryonic and fetal testes and ovaries, both at gene transcript and protein level. A higher expression of IGF regulators was detected in testes than ovaries, with a preferred localization to Leydig cells.

CONCLUSIONS: These data indicate that the IGF system is active during very early gestation, when it may have a regulatory role in Leydig cells.

OriginalsprogEngelsk
TidsskriftThe Journal of clinical endocrinology and metabolism
Vol/bind105
Udgave nummer9
Sider (fra-til)E3157-E3168
ISSN0021-972X
DOI
StatusUdgivet - 1 sep. 2020

Bibliografisk note

© Endocrine Society 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

ID: 61252714