@article{5efd98b073bf11dfa4a6000ea68e967b,
title = "Expression of BK Ca channels and the modulatory beta-subunits in the rat and porcine trigeminal ganglion",
abstract = "Large conductance calcium-activated potassium (BK(Ca)) channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release. Migraine has been proposed to be a trigeminovascular disease involving the sensory trigeminal pathways and the cerebral arteries. We hypothesize that BK(Ca) channel alpha- and beta-subunits are present in the rat and porcine trigeminal ganglion (TG) thus enabling a role in migraine. BK(Ca) channel mRNA was detected using reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization. BK(Ca) channel protein was visualized by western blotting and histochemistry. The presence of the modulatory beta1-beta 4 subunit mRNAs was investigated using RT-PCR. beta1-, beta2- and beta 4-subunit mRNAs were expressed in rat TG whereas beta2- and beta 4-subunits were detected in porcine TG. Western blotting revealed beta2- and beta 4-subunit proteins in rat and porcine TG. The present study showed BK(Ca) channel expression in rat and porcine TG. The main modulatory beta-subunits detected in TG of both species were beta2- and beta 4-subunits.",
author = "Johansson, {Helle Wulf} and Anders Hay-Schmidt and Poulsen, {Asser Nyander} and Kl{\ae}rke, {Dan Arne} and Jes Olesen and Inger Jansen-Olesen",
note = "Keywords: Animals; Blotting, Western; Immunoblotting; In Situ Hybridization; Large-Conductance Calcium-Activated Potassium Channel beta Subunits; Large-Conductance Calcium-Activated Potassium Channels; Neurons; Photomicrography; RNA, Messenger; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Species Specificity; Swine; Trigeminal Ganglion; Xenopus",
year = "2009",
doi = "10.1016/j.brainres.2009.07.047",
language = "English",
volume = "1292",
pages = "1--13",
journal = "Brain Research",
issn = "1872-6240",
publisher = "Elsevier BV",
}