Expression and crystallographic studies of the D1D2 domains of C4.4A, a homologous protein to the urokinase receptor

Shanli Chen, Lin Lin, Cai Yuan, Henrik Gårdsvoll, Mette C Kriegbaum, Michael Ploug, Mingdong Huang

    1 Citationer (Scopus)

    Abstract

    C4.4A is a glycosylphosphatidylinositol-anchored membrane protein comprised of two LU domains (Ly6/uPAR-like domains) and an extensively O-glycosylated C-terminal Ser/Thr/Pro-rich region. C4.4A is a novel biomarker for squamous epithelial differentiation. Its expression is dysregulated under various pathological conditions and it is a robust biomarker for poor prognosis in various malignant conditions such as pulmonary adenocarcinoma. To facilitate crystallization, the two LU domains were excised from intact C4.4A by limited proteolysis, purified and crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to 2.7 Å resolution and belonged to space group C2221, with unit-cell parameters a = 55.49, b = 119.63, c = 168.54 Å. The statistics indicated good quality of the data, which form a solid basis for the determination of the C4.4A structure.

    OriginalsprogEngelsk
    TidsskriftActa crystallographica. Section F, Structural biology communications
    Vol/bind73
    Udgave nummerPt 8
    Sider (fra-til)486-490
    Antal sider5
    ISSN2053-230X
    DOI
    StatusUdgivet - 1 aug. 2017

    Fingeraftryk

    Dyk ned i forskningsemnerne om 'Expression and crystallographic studies of the D1D2 domains of C4.4A, a homologous protein to the urokinase receptor'. Sammen danner de et unikt fingeraftryk.

    Citationsformater