Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin

Manoj Puthia*, Lloyd Tanner, Ganna Petruk, Artur Schmidtchen

*Corresponding author af dette arbejde
11 Citationer (Scopus)

Abstract

COVID-19 is characterized by a dysregulated and excessive inflammatory response and, in severe cases, acute respiratory distress syndrome. We have recently demonstrated a previously unknown high-affinity interaction between the SARS-CoV-2 spike (S) protein and bacterial lipopolysaccharide (LPS), leading to the boosting of inflammation. Here we present a mouse inflammation model employing the coadministration of aerosolized S protein together with LPS to the lungs. Using NF-κB-RE-Luc reporter and C57BL/6 mice followed by combinations of bioimaging, cytokine, chemokine, fluorescence-activated cell sorting, and histochemistry analyses, we show that the model yields severe pulmonary inflammation and a cytokine profile similar to that observed in COVID-19. Therefore, the model offers utility for analyses of the pathophysiological features of COVID-19 and the development of new treatments.

OriginalsprogEngelsk
TidsskriftACS Pharmacology and Translational Science
Vol/bind5
Udgave nummer3
Sider (fra-til)141-148
Antal sider8
ISSN2575-9108
DOI
StatusUdgivet - 11 mar. 2022

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