Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
E-pub ahead of print

Exome sequencing of 22 genes using tissue from patients with biliary tract cancer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


  1. A case of false-positive pneumococcal urinary antigen test in a bacteremic Streptococcus agalactiae infection

    Publikation: Bidrag til tidsskriftLetterForskningpeer review

  2. The environmental occurrence of Pseudomonas aeruginosa

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Bacteremia and urogenital infection with Actinomyces urogenitalis following prolonged urinary retention

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Shared heritability and functional enrichment across six solid cancers

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

BACKGROUND: Biliary tract cancers (BTC) are a rare heterogeneous disease group with a dismal prognosis and limited treatment options. The mutational landscape consists of genetic aberrations both shared by and characteristic for anatomical location. Here we present exome sequencing data on 22 genes from a phase 2 trial using a clinically validated panel used in patients with colorectal cancer.

METHODS: A total of 56 patients were included in a one-armed phase 2 trial investigating the treatment combination of capecitabine, gemcitabine, oxaliplatin and cetuximab. Tissue DNA yield and quality allowed analysis of 30 patients on our panel including 22 genes.

RESULTS: ARID1A (33%) and TP53 (33%) were found to be most frequently mutated followed by KRAS mutations found in 20% of the patients. Mutational aberrations in ARID1A were found more prevalent than expected, whereas TP53 and KRAS where in concordance with earlier reported data. Mutation in CTNNB1 was significantly associated with poor prognosis.

CONCLUSION: Our panel is clinically validated and suitable for a high volume of samples to detect mutations in patients with BTC. However, it is reasonable to assume that the clinical utility could be optimized in this patient group by extending the panel to include BTC specific mutations with potential therapeutic consequences such as IDH1/2, FGFR-fusions, ERBB3 and BRCA1/2.

TidsskriftAPMIS - Journal of Pathology, Microbiology and Immunology
StatusE-pub ahead of print - 19 okt. 2019

Bibliografisk note

© 2019 APMIS. Published by John Wiley & Sons Ltd.

ID: 58190675