Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Evolution of the Pseudomonas aeruginosa mutational resistome in an international Cystic Fibrosis clone

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Identification of two different coagulation phenotypes in people living with HIV with undetectable viral replication

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Developing and validating COVID-19 adverse outcome risk prediction models from a bi-national European cohort of 5594 patients

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Detection of biological signals from a live mammalian muscle using an early stage diamond quantum sensor

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, "Silenusvirus"

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. The effect of diabetes and the common diabetogenic TBC1D4 p.Arg684Ter variant on cardiovascular risk in Inuit in Greenland

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Bacterial persisters in long-term infection: Emergence and fitness in a complex host environment

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Gene Loss and Acquisition in Lineages of Pseudomonas aeruginosa Evolving in Cystic Fibrosis Patient Airways

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. A new device to prevent contamination of nasal swabs by Staphylococcus aureus in acute rhinosinuitis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Antibiotic resistance in Pseudomonas aeruginosa and adaptation to complex dynamic environments

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  5. Microcontainer Delivery of Antibiotic Improves Treatment of Pseudomonas aeruginosa Biofilms

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Emergence of epidemic clones and antibiotic resistance development compromises the management of Pseudomonas aeruginosa cystic fibrosis (CF) chronic respiratory infections. Whole genome sequencing (WGS) was used to decipher the phylogeny, interpatient dissemination, WGS mutator genotypes (mutome) and resistome of a widespread clone (CC274), in isolates from two highly-distant countries, Australia and Spain, covering an 18-year period. The coexistence of two divergent CC274 clonal lineages was revealed, but without evident geographical barrier; phylogenetic reconstructions and mutational resistome demonstrated the interpatient transmission of mutators. The extraordinary capacity of P. aeruginosa to develop resistance was evidenced by the emergence of mutations in >100 genes related to antibiotic resistance during the evolution of CC274, catalyzed by mutator phenotypes. While the presence of classical mutational resistance mechanisms was confirmed and correlated with resistance phenotypes, results also showed a major role of unexpected mutations. Among them, PBP3 mutations, shaping up β-lactam resistance, were noteworthy. A high selective pressure for mexZ mutations was evidenced, but we showed for the first time that high-level aminoglycoside resistance in CF is likely driven by mutations in fusA1/fusA2, coding for elongation factor G. Altogether, our results provide valuable information for understanding the evolution of the mutational resistome of CF P. aeruginosa.

OriginalsprogEngelsk
TidsskriftScientific Reports
Vol/bind7
Udgave nummer1
Sider (fra-til)5555
ISSN2045-2322
DOI
StatusUdgivet - 17 jul. 2017

ID: 52702222