Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Evolution of the β-lactam-resistant Streptococcus pneumoniae PMEN3 clone over a 30 year period in Barcelona, Spain

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Harvard

Càmara, J, Cubero, M, Martín-Galiano, AJ, García, E, Grau, I, Nielsen, JB, Worning, P, Tubau, F, Pallarés, R, Domínguez, MÁ, Kilian, M, Liñares, J, Westh, H & Ardanuy, C 2018, 'Evolution of the β-lactam-resistant Streptococcus pneumoniae PMEN3 clone over a 30 year period in Barcelona, Spain' Journal of Antimicrobial Chemotherapy, bind 73, nr. 11, s. 2941-2951. https://doi.org/10.1093/jac/dky305

APA

CBE

Càmara J, Cubero M, Martín-Galiano AJ, García E, Grau I, Nielsen JB, Worning P, Tubau F, Pallarés R, Domínguez MÁ, Kilian M, Liñares J, Westh H, Ardanuy C. 2018. Evolution of the β-lactam-resistant Streptococcus pneumoniae PMEN3 clone over a 30 year period in Barcelona, Spain. Journal of Antimicrobial Chemotherapy. 73(11):2941-2951. https://doi.org/10.1093/jac/dky305

MLA

Vancouver

Author

Càmara, Jordi ; Cubero, Meritxell ; Martín-Galiano, Antonio J ; García, Ernesto ; Grau, Imma ; Nielsen, Jesper B ; Worning, Peder ; Tubau, Fe ; Pallarés, Román ; Domínguez, M Ángeles ; Kilian, Mogens ; Liñares, Josefina ; Westh, Henrik ; Ardanuy, Carmen. / Evolution of the β-lactam-resistant Streptococcus pneumoniae PMEN3 clone over a 30 year period in Barcelona, Spain. I: Journal of Antimicrobial Chemotherapy. 2018 ; Bind 73, Nr. 11. s. 2941-2951.

Bibtex

@article{fc31eac07c9b454f80d500a7c82adeb1,
title = "Evolution of the β-lactam-resistant Streptococcus pneumoniae PMEN3 clone over a 30 year period in Barcelona, Spain",
abstract = "Objectives: To analyse the epidemiology and genetic evolution of PMEN3 (Spain9V-156), a penicillin-non-susceptible clone of Streptococcus pneumoniae, causing invasive pneumococcal disease (IPD) in Barcelona during 1987-2016.Methods: WGS was performed on 46 representative isolates and the data were used to design additional molecular typing methods including partial MLST, PCR-RFLP and detection of surface-exposed proteins and prophages, to assign the remaining isolates to lineages. The isolates were also subjected to antimicrobial susceptibility testing.Results: Two hundred and twenty-seven adult cases of IPD caused by PMEN3 were identified. PMEN3 caused mainly pneumonia (84{\%}) and the 30 day mortality rate was 23.1{\%}. Evidence of recombination events was found, mostly in three regions, namely the capsular operon (associated with capsular switching) and adjacent regions containing pbp2x and pbp1a, the murM gene and the pbp2b-ddl region. Some of these genetic changes generated successful new variant serotype lineages, including one of serotype 11A that is not included in the current PCV13 vaccine. Other genetic changes led to increased MICs of β-lactams. Notably, most isolates also harboured prophages coding for PblB-like proteins. Despite these adaptations, the ability of this clone to cause IPD remained unchanged over time, highlighting the importance of its core genetic background.Conclusions: Our study demonstrated successful adaptation of PMEN3 to persist over time despite the introduction of broader antibiotics and conjugate vaccines. In addition to enhancing understanding of the molecular evolution of PMEN3, these findings highlight the need for the development of non-serotype-based vaccines to fight pneumococcal infection.",
author = "Jordi C{\`a}mara and Meritxell Cubero and Mart{\'i}n-Galiano, {Antonio J} and Ernesto Garc{\'i}a and Imma Grau and Nielsen, {Jesper B} and Peder Worning and Fe Tubau and Rom{\'a}n Pallar{\'e}s and Dom{\'i}nguez, {M {\'A}ngeles} and Mogens Kilian and Josefina Li{\~n}ares and Henrik Westh and Carmen Ardanuy",
year = "2018",
month = "11",
doi = "10.1093/jac/dky305",
language = "English",
volume = "73",
pages = "2941--2951",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "11",

}

RIS

TY - JOUR

T1 - Evolution of the β-lactam-resistant Streptococcus pneumoniae PMEN3 clone over a 30 year period in Barcelona, Spain

AU - Càmara, Jordi

AU - Cubero, Meritxell

AU - Martín-Galiano, Antonio J

AU - García, Ernesto

AU - Grau, Imma

AU - Nielsen, Jesper B

AU - Worning, Peder

AU - Tubau, Fe

AU - Pallarés, Román

AU - Domínguez, M Ángeles

AU - Kilian, Mogens

AU - Liñares, Josefina

AU - Westh, Henrik

AU - Ardanuy, Carmen

PY - 2018/11

Y1 - 2018/11

N2 - Objectives: To analyse the epidemiology and genetic evolution of PMEN3 (Spain9V-156), a penicillin-non-susceptible clone of Streptococcus pneumoniae, causing invasive pneumococcal disease (IPD) in Barcelona during 1987-2016.Methods: WGS was performed on 46 representative isolates and the data were used to design additional molecular typing methods including partial MLST, PCR-RFLP and detection of surface-exposed proteins and prophages, to assign the remaining isolates to lineages. The isolates were also subjected to antimicrobial susceptibility testing.Results: Two hundred and twenty-seven adult cases of IPD caused by PMEN3 were identified. PMEN3 caused mainly pneumonia (84%) and the 30 day mortality rate was 23.1%. Evidence of recombination events was found, mostly in three regions, namely the capsular operon (associated with capsular switching) and adjacent regions containing pbp2x and pbp1a, the murM gene and the pbp2b-ddl region. Some of these genetic changes generated successful new variant serotype lineages, including one of serotype 11A that is not included in the current PCV13 vaccine. Other genetic changes led to increased MICs of β-lactams. Notably, most isolates also harboured prophages coding for PblB-like proteins. Despite these adaptations, the ability of this clone to cause IPD remained unchanged over time, highlighting the importance of its core genetic background.Conclusions: Our study demonstrated successful adaptation of PMEN3 to persist over time despite the introduction of broader antibiotics and conjugate vaccines. In addition to enhancing understanding of the molecular evolution of PMEN3, these findings highlight the need for the development of non-serotype-based vaccines to fight pneumococcal infection.

AB - Objectives: To analyse the epidemiology and genetic evolution of PMEN3 (Spain9V-156), a penicillin-non-susceptible clone of Streptococcus pneumoniae, causing invasive pneumococcal disease (IPD) in Barcelona during 1987-2016.Methods: WGS was performed on 46 representative isolates and the data were used to design additional molecular typing methods including partial MLST, PCR-RFLP and detection of surface-exposed proteins and prophages, to assign the remaining isolates to lineages. The isolates were also subjected to antimicrobial susceptibility testing.Results: Two hundred and twenty-seven adult cases of IPD caused by PMEN3 were identified. PMEN3 caused mainly pneumonia (84%) and the 30 day mortality rate was 23.1%. Evidence of recombination events was found, mostly in three regions, namely the capsular operon (associated with capsular switching) and adjacent regions containing pbp2x and pbp1a, the murM gene and the pbp2b-ddl region. Some of these genetic changes generated successful new variant serotype lineages, including one of serotype 11A that is not included in the current PCV13 vaccine. Other genetic changes led to increased MICs of β-lactams. Notably, most isolates also harboured prophages coding for PblB-like proteins. Despite these adaptations, the ability of this clone to cause IPD remained unchanged over time, highlighting the importance of its core genetic background.Conclusions: Our study demonstrated successful adaptation of PMEN3 to persist over time despite the introduction of broader antibiotics and conjugate vaccines. In addition to enhancing understanding of the molecular evolution of PMEN3, these findings highlight the need for the development of non-serotype-based vaccines to fight pneumococcal infection.

U2 - 10.1093/jac/dky305

DO - 10.1093/jac/dky305

M3 - Journal article

VL - 73

SP - 2941

EP - 2951

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 11

ER -

ID: 55129465