TY - JOUR
T1 - Evaluation of the Merlin, Micronaut system for automated antimicrobial susceptibility testing of Pseudomonas aeruginosa and Burkholderia species isolated from cystic fibrosis patients
AU - Häussler, S
AU - Ziesing, S
AU - Rademacher, G
AU - Hoy, L
AU - Weissbrodt, H
PY - 2003/8
Y1 - 2003/8
N2 - Since accurate antimicrobial susceptibility testing of bacterial cystic fibrosis isolates is known to be problematic and an optimal in vitro testing method has not yet been evaluated, the study presented here was conducted to compare the performance of the reference agar dilution method and broth microdilution with a commercially available automated susceptibility test system (Merlin; Micronaut, Germany). In this pilot study, the susceptibility of 70 clinical strains of Pseudomonas aeruginosa and Burkholderia cepacia-like organisms to nine antimicrobial agents was tested using these methods. Susceptibility results generated by broth microdilution (both automated and according to the National Committee for Clinical Laboratory Standards recommendations) were demonstrated to be of good reproducibility, and they compared favourably to the time- and material-consuming standard agar dilution reference method, especially after a prolonged incubation time (48 h).
AB - Since accurate antimicrobial susceptibility testing of bacterial cystic fibrosis isolates is known to be problematic and an optimal in vitro testing method has not yet been evaluated, the study presented here was conducted to compare the performance of the reference agar dilution method and broth microdilution with a commercially available automated susceptibility test system (Merlin; Micronaut, Germany). In this pilot study, the susceptibility of 70 clinical strains of Pseudomonas aeruginosa and Burkholderia cepacia-like organisms to nine antimicrobial agents was tested using these methods. Susceptibility results generated by broth microdilution (both automated and according to the National Committee for Clinical Laboratory Standards recommendations) were demonstrated to be of good reproducibility, and they compared favourably to the time- and material-consuming standard agar dilution reference method, especially after a prolonged incubation time (48 h).
KW - Anti-Bacterial Agents/pharmacology
KW - Automation
KW - Bacteriological Techniques
KW - Burkholderia/drug effects
KW - Cystic Fibrosis/drug therapy
KW - Drug Resistance, Bacterial
KW - Female
KW - Humans
KW - Male
KW - Microbial Sensitivity Tests
KW - Pseudomonas Infections/diagnosis
KW - Pseudomonas aeruginosa/drug effects
KW - Sensitivity and Specificity
U2 - 10.1007/s10096-003-0974-7
DO - 10.1007/s10096-003-0974-7
M3 - Journal article
C2 - 12898284
SN - 0934-9723
VL - 22
SP - 496
EP - 500
JO - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
IS - 8
ER -