Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Evaluation of a New Automated Routine Measurement for Serum Adjusted Ionized Calcium (at pH 7.4) in Patients Suspected of Calcium Metabolic Disease

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. MR-proANP associated with body mass index in patients with anorexia nervosa

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Early and Accurate Sex Dtermination by qPCR of Y Chromosome Repetitive Sequence (YRS) In Cell-Free Fetal DNA from Maternal Plasma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Evaluation of the HemoCue WBC DIFF in leukopenic patient samples

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. A case of reassortant seasonal influenza A(H1N2) virus, Denmark, April 2019

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Feasibility of single-cell analysis of model cancer and foetal cells in blood after isolation by cell picking

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

BACKGROUND: Total calcium is a less accurate test in predicting ionized calcium (Ca2+) in patients suspected of calcium metabolic disease. Nevertheless, total calcium continues to be used as routine measurement instead of adjusted Ca2+ (at pH 7.4). In the current study we evaluate a new multichannel instrument, the ISE Module E1200 for adjusted Ca2+ (at pH 7.4), containing three different ion-selective electrode (ISE) units.

METHODS: Serum from 1350 patients was compared to the ABL835 flex and KoneLab. Total calcium was also evaluated on the Dimension Vista 1500 system. Correlations between instruments were assessed by Deming regression and degree of agreement by Cohen's kappa (κ).

RESULTS: Analytical imprecisions for the three ISE units for adjusted Ca2+ (at pH 7.4) was between 0.36% and 2.52%, and for pH between 0.32% and 3.24%. Results were comparable for each ISE unit (r = 0.797-0.917; all P < 0.0001) and in high-throughput settings (r = 0.871; P < 0.0001). The degree of agreement between instruments was moderate to good (κ  =  0.52-0.77). In contrast, there was a very poor agreement (κ = -0.14) for total calcium with discrepancy in 53.4% of the samples.

CONCLUSIONS: The new ISE Module E1200 is comparable with the ABL835 flex and KoneLab 30i and therefore may be used for routine analysis of serum adjusted Ca2+ (at pH 7.4). The measured adjusted Ca2+ (at pH 7.4) was less comparable with very poor agreement to total calcium measured on the Dimension Vista 1500 system.

OriginalsprogEngelsk
TidsskriftThe Journal of Applied Laboratory Medicine
Vol/bind5
Udgave nummer4
Sider (fra-til)704-715
Antal sider12
ISSN2576-9456
DOI
StatusUdgivet - 1 jul. 2020

Bibliografisk note

© American Association for Clinical Chemistry 2020.

ID: 60232236