Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

European external quality assessments for identification, molecular typing and characterization of Staphylococcus aureus

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Point of care testing for infectious disease: ownership and quality

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. ResFinder 4.0 for predictions of phenotypes from genotypes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures: Validation in 55 european laboratories

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Staphylococcus saprophyticus Causing Infections in Humans Is Associated with High Resistance to Heavy Metals

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Staphylococcus saprophyticus From Clinical and Environmental Origins Have Distinct Biofilm Composition

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Rapid Open-Source SNP-Based Clustering Offers an Alternative to Core Genome MLST for Outbreak Tracing in a Hospital Setting

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Study Group on Staphylococci and Staphylococcal Infections (ESGS) of ESCMID
Vis graf over relationer

Objectives: We present the results of two European external quality assessments (EQAs) conducted in 2014 and 2016 under the auspices of the Study Group on Staphylococci and Staphylococcal Infections of ESCMID. The objective was to assess the performance of participating centres in characterizing Staphylococcus aureus using their standard in-house phenotypic and genotypic protocols.

Methods: A total of 11 well-characterized blindly coded S. aureus (n = 9), Staphylococcus argenteus (n = 1) and Staphylococcus capitis (n = 1) strains were distributed to participants for analysis. Species identification, MIC determination, antimicrobial susceptibility testing, antimicrobial resistance and toxin gene detection and molecular typing including spa typing, SCCmec typing and MLST were performed.

Results: Thirteen laboratories from 12 European countries participated in one EQA or both EQAs. Despite considerable diversity in the methods employed, good concordance (90%-100%) with expected results was obtained. Discrepancies were observed for: (i) identification of the S. argenteus strain; (ii) phenotypic detection of low-level resistance to oxacillin in the mecC-positive strain; (iii) phenotypic detection of the inducible MLSB strain; and (iv) WGS-based detection of some resistance and toxin genes.

Conclusions: Overall, good concordance (90%-100%) with expected results was observed. In some instances, the accurate detection of resistance and toxin genes from WGS data proved problematic, highlighting the need for validated and internationally agreed-on bioinformatics pipelines before such techniques are implemented routinely by microbiology laboratories. We strongly recommend all national reference laboratories and laboratories acting as referral centres to participate in such EQA initiatives.

OriginalsprogEngelsk
TidsskriftThe Journal of antimicrobial chemotherapy
Vol/bind73
Udgave nummer10
Sider (fra-til)2662-2666
ISSN0305-7453
DOI
StatusUdgivet - 1 okt. 2018

ID: 54974990