Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Erythrocytes restrict microvesicle-induced production of reactive oxygen species by polymorphonuclear leukocytes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Bacteremia and urogenital infection with Actinomyces urogenitalis following prolonged urinary retention

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Two cases of tick-borne transmitted tularemia on Southern Zealand, Denmark

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Analysis of proximal bone margins in diabetic foot osteomyelitis by conventional culture, DNA sequencing and microscopy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. L-serine: a neglected amino acid with a potential therapeutic role in diabetes

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  5. The impact of concurrent HIV and type II diabetes on immune maturation, immune regulation and immune activation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Peripheral Nervous System Disease in Systemic Lupus Erythematosus: Results From an International Inception Cohort Study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Prognostic role of genetic polymorphisms of the interleukin-6 signaling pathway in patients with severe heart failure

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Thyroid peroxidase antibodies and prospective live birth rate: A cohort study of women with recurrent pregnancy loss

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Microvesicles (MVs) are extracellular vesicles released by several cell types upon activation or apoptosis. MVs have the potential to activate complement, which has been suggested to mediate their clearance. However, it is not clear how complement-opsonized MVs are prevented from activating circulating polymorphonuclear leukocytes (PMNs) with release of reactive oxygen species (ROS) and potential damage of endothelium and other bystander cells as consequence. We hypothesized that binding of opsonized MVs to erythrocytes (Es) attenuates MV-induced PMN activation. To test this, normal PMNs were exposed to MVs in the presence and absence of Es from allogenic healthy donors. As analyzed by flow cytometry, the presence of Es restricted the PMN binding of MVs by about 85% (p = 0.002) and mediated a 60-70% inhibition of the PMN production of the ROS H2 O2 , induced by MVs, when lipopolysaccharide was used as a primer (p = 0.002). The competitive binding of MVs to Es was partly dependent on complement, since EDTA inhibited MV binding to Es by 75%. These data suggest that Es, through competitive binding, may restrict MV-induced activation of circulating PMNs and thereby serve a role as a regulator of PMN activation.

OriginalsprogEngelsk
TidsskriftAPMIS - Journal of Pathology, Microbiology and Immunology
Vol/bind127
Udgave nummer7
Sider (fra-til)538-542
Antal sider5
ISSN0903-4641
DOI
StatusUdgivet - jul. 2019

Bibliografisk note

© 2019 APMIS. Published by John Wiley & Sons Ltd.

ID: 58901428