Eosinophilic airway inflammation in asthma is associated with an altered airway microbiome

Asger Sverrild; Pia Kiilerich; Asker Brejnrod; Rebecca Pedersen; Celeste Porsbjerg; Anders Bergqvist; Jonas S Erjefält; Karsten Kristiansen; Vibeke Backer

doi:10.1016/j.jaci.2016.10.046

Eosinophilic airway inflammation in asthma is associated with an altered airway microbiome

Asger Sverrild, Pia Kiilerich, Asker Brejnrod, Rebecca Pedersen, Celeste Porsbjerg, Anders Bergqvist, Jonas S Erjefält, Karsten Kristiansen, Vibeke Backer

Lungemedicinsk Afd. L

95 Citationer (Scopus)

Abstract

BACKGROUND: Subjects with asthma have higher microbiome diversity, and an altered composition with more Proteobacteria and less Bacteroidetes compared to healthy controls. Studies comparing airway inflammation and airway microbiome are sparse, especially in subjects not on anti-inflammatory treatment.

OBJECTIVE: To describe the relationship between the airway microbiome and patterns of airway inflammation in steroid-free subjects with asthma and healthy controls.

METHODS: Broncho-alveolar lavage was collected from 23 steroid-free, non-smoking subjects with asthma and 10 healthy controls. Bacterial DNA was extracted from and subjected to Illumina MiSeq sequencing of the 16S rDNA V4 region. Eosinophils and neutrophils in submucosa were quantified by immunohistochemical identification and computerized image analysis. Induced sputum was performed, and Airway hyperresponsiveness to mannitol and Fractional Exhaled Nitric Oxide were measured. Relationships between airway microbial diversity and composition, and inflammatory profiles were analyzed.

RESULTS: In patients with asthma, airway microbial composition was associated with airway eosinophilia and AHR to mannitol, but not airway neutrophilia. The overall composition of the airway microbiome of asthmatics with the lowest levels of eosinophils, but not asthmatics with the highest levels of eosinophils deviated significantly from that of healthy individuals. Asthmatics with the lowest levels of eosinophils had an altered bacterial abundance profile with more Neisseria, Bacteroides and Rothia, and less Sphingomonas, Halomonas and Aeribacillus compared to asthmatics with more eosinophils and healthy controls.

CONCLUSION: The level of eosinophilic airway inflammation correlates with variations in microbiomes across subjects with asthma, whereas neutrophilic airway inflammation does not. This warrants further investigation on molecular pathways involved in eosinophilic as well as non-eosinophilic asthma.

Originalsprog	Engelsk
Tidsskrift	The Journal of allergy and clinical immunology
Vol/bind	140
Udgave nummer	2
Sider (fra-til)	407-417
Antal sider	11
ISSN	0091-6749
DOI	https://doi.org/10.1016/j.jaci.2016.10.046
Status	Udgivet - aug. 2017

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10.1016/j.jaci.2016.10.046

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title = "Eosinophilic airway inflammation in asthma is associated with an altered airway microbiome",

abstract = "BACKGROUND: Subjects with asthma have higher microbiome diversity, and an altered composition with more Proteobacteria and less Bacteroidetes compared to healthy controls. Studies comparing airway inflammation and airway microbiome are sparse, especially in subjects not on anti-inflammatory treatment.OBJECTIVE: To describe the relationship between the airway microbiome and patterns of airway inflammation in steroid-free subjects with asthma and healthy controls.METHODS: Broncho-alveolar lavage was collected from 23 steroid-free, non-smoking subjects with asthma and 10 healthy controls. Bacterial DNA was extracted from and subjected to Illumina MiSeq sequencing of the 16S rDNA V4 region. Eosinophils and neutrophils in submucosa were quantified by immunohistochemical identification and computerized image analysis. Induced sputum was performed, and Airway hyperresponsiveness to mannitol and Fractional Exhaled Nitric Oxide were measured. Relationships between airway microbial diversity and composition, and inflammatory profiles were analyzed.RESULTS: In patients with asthma, airway microbial composition was associated with airway eosinophilia and AHR to mannitol, but not airway neutrophilia. The overall composition of the airway microbiome of asthmatics with the lowest levels of eosinophils, but not asthmatics with the highest levels of eosinophils deviated significantly from that of healthy individuals. Asthmatics with the lowest levels of eosinophils had an altered bacterial abundance profile with more Neisseria, Bacteroides and Rothia, and less Sphingomonas, Halomonas and Aeribacillus compared to asthmatics with more eosinophils and healthy controls.CONCLUSION: The level of eosinophilic airway inflammation correlates with variations in microbiomes across subjects with asthma, whereas neutrophilic airway inflammation does not. This warrants further investigation on molecular pathways involved in eosinophilic as well as non-eosinophilic asthma.",

author = "Asger Sverrild and Pia Kiilerich and Asker Brejnrod and Rebecca Pedersen and Celeste Porsbjerg and Anders Bergqvist and Erjef{\"a}lt, {Jonas S} and Karsten Kristiansen and Vibeke Backer",

note = "Copyright {\textcopyright} 2016. Published by Elsevier Inc.",

year = "2017",

month = aug,

doi = "10.1016/j.jaci.2016.10.046",

language = "English",

volume = "140",

pages = "407--417",

journal = "The Journal of allergy and clinical immunology",

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publisher = "Elsevier Inc.",

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TY - JOUR

T1 - Eosinophilic airway inflammation in asthma is associated with an altered airway microbiome

AU - Sverrild, Asger

AU - Kiilerich, Pia

AU - Brejnrod, Asker

AU - Pedersen, Rebecca

AU - Porsbjerg, Celeste

AU - Bergqvist, Anders

AU - Erjefält, Jonas S

AU - Kristiansen, Karsten

AU - Backer, Vibeke

PY - 2017/8

Y1 - 2017/8

N2 - BACKGROUND: Subjects with asthma have higher microbiome diversity, and an altered composition with more Proteobacteria and less Bacteroidetes compared to healthy controls. Studies comparing airway inflammation and airway microbiome are sparse, especially in subjects not on anti-inflammatory treatment.OBJECTIVE: To describe the relationship between the airway microbiome and patterns of airway inflammation in steroid-free subjects with asthma and healthy controls.METHODS: Broncho-alveolar lavage was collected from 23 steroid-free, non-smoking subjects with asthma and 10 healthy controls. Bacterial DNA was extracted from and subjected to Illumina MiSeq sequencing of the 16S rDNA V4 region. Eosinophils and neutrophils in submucosa were quantified by immunohistochemical identification and computerized image analysis. Induced sputum was performed, and Airway hyperresponsiveness to mannitol and Fractional Exhaled Nitric Oxide were measured. Relationships between airway microbial diversity and composition, and inflammatory profiles were analyzed.RESULTS: In patients with asthma, airway microbial composition was associated with airway eosinophilia and AHR to mannitol, but not airway neutrophilia. The overall composition of the airway microbiome of asthmatics with the lowest levels of eosinophils, but not asthmatics with the highest levels of eosinophils deviated significantly from that of healthy individuals. Asthmatics with the lowest levels of eosinophils had an altered bacterial abundance profile with more Neisseria, Bacteroides and Rothia, and less Sphingomonas, Halomonas and Aeribacillus compared to asthmatics with more eosinophils and healthy controls.CONCLUSION: The level of eosinophilic airway inflammation correlates with variations in microbiomes across subjects with asthma, whereas neutrophilic airway inflammation does not. This warrants further investigation on molecular pathways involved in eosinophilic as well as non-eosinophilic asthma.

AB - BACKGROUND: Subjects with asthma have higher microbiome diversity, and an altered composition with more Proteobacteria and less Bacteroidetes compared to healthy controls. Studies comparing airway inflammation and airway microbiome are sparse, especially in subjects not on anti-inflammatory treatment.OBJECTIVE: To describe the relationship between the airway microbiome and patterns of airway inflammation in steroid-free subjects with asthma and healthy controls.METHODS: Broncho-alveolar lavage was collected from 23 steroid-free, non-smoking subjects with asthma and 10 healthy controls. Bacterial DNA was extracted from and subjected to Illumina MiSeq sequencing of the 16S rDNA V4 region. Eosinophils and neutrophils in submucosa were quantified by immunohistochemical identification and computerized image analysis. Induced sputum was performed, and Airway hyperresponsiveness to mannitol and Fractional Exhaled Nitric Oxide were measured. Relationships between airway microbial diversity and composition, and inflammatory profiles were analyzed.RESULTS: In patients with asthma, airway microbial composition was associated with airway eosinophilia and AHR to mannitol, but not airway neutrophilia. The overall composition of the airway microbiome of asthmatics with the lowest levels of eosinophils, but not asthmatics with the highest levels of eosinophils deviated significantly from that of healthy individuals. Asthmatics with the lowest levels of eosinophils had an altered bacterial abundance profile with more Neisseria, Bacteroides and Rothia, and less Sphingomonas, Halomonas and Aeribacillus compared to asthmatics with more eosinophils and healthy controls.CONCLUSION: The level of eosinophilic airway inflammation correlates with variations in microbiomes across subjects with asthma, whereas neutrophilic airway inflammation does not. This warrants further investigation on molecular pathways involved in eosinophilic as well as non-eosinophilic asthma.

U2 - 10.1016/j.jaci.2016.10.046

DO - 10.1016/j.jaci.2016.10.046

M3 - Journal article

C2 - 28042058

SN - 0091-6749

VL - 140

SP - 407

EP - 417

JO - The Journal of allergy and clinical immunology

JF - The Journal of allergy and clinical immunology

IS - 2

ER -

Eosinophilic airway inflammation in asthma is associated with an altered airway microbiome

Abstract

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