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Region Hovedstaden - en del af Københavns Universitetshospital
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Enzyme-linked immunosorbent serum assay specific for the 7S domain of Collagen Type IV (P4NP 7S): A marker related to the extracellular matrix remodeling during liver fibrogenesis

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  • Diana Julie Leeming
  • Mette J Nielsen
  • Yueqin Dai
  • Sanne Skovgård Veidal
  • Efstathios Vassiliadis
  • Chen Zhang
  • Yi He
  • Ben Vainer
  • Qinlong Zheng
  • Morten A Karsdal
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Aim:  The present study describes the ability of a newly developed N-terminal pro-peptides of type IV collagen 7S domain (P4NP 7S) competitive enzyme-linked immunosorbent assay (ELISA) for describing liver fibrosis. The assay applies a monoclonal antibody specific for a PIVNP 7S epitope 100% homologous in the human, rat, and mouse species. Methods:  Monoclonal antibodies were raised against selected P4NP 7S specific sequences. Antibodies were screened and a competitive ELISA assay was developed using a selected antibody. The assay was evaluated in relation to technical performance, and in two preclinical liver fibrosis models; the bile duct ligation model (BDL) and the carbon tetrachloride model (CCL4) both performed in rats. Results:  A technically robust P4NP 7S ELISA assay using a monoclonal antibody was produced. In the BDL and CCL4 liver fibrosis models it was observed that the P4NP 7S levels were significantly elevated in rat with liver fibrosis as seen by histology (CCL4: 283% elevated in the highest quartile of total hepatic collagen compared with controls, P = 0.001; BDL: 183% elevated at week 4 compared with sham, P 
OriginalsprogEngelsk
TidsskriftHepatology Research
Vol/bind42
Udgave nummer5
Sider (fra-til)482-93
Antal sider12
ISSN1386-6346
DOI
StatusUdgivet - 2012

ID: 36590228