TY - JOUR
T1 - Enzyme-linked immunosorbent assay for determination of thyroglobulin autoantibodies. Comparison with RIA and haemagglutination
AU - Høier-Madsen, M
AU - Feldt-Rasmussen, U
AU - Hegedüs, L
AU - Perrild, H
AU - Hansen, H S
PY - 1984/12
Y1 - 1984/12
N2 - An enzyme immunoassay (ELISA) has been developed for the detection of human autoantibodies to thyroglobulin (TgAb). The ELISA assay was compared to the previous routine method, the tanned red cell (TRC) method and a radioimmunoassay (RIA) technique for thyroglobulin autoantibodies. Sera from 163 healthy non-hospitalized persons, 31 patients with Hashimoto's thyroiditis, 13 patients with Graves disease, 136 patients with thyroid cancer and 365 sera consecutively received for thyroid autoantibody screening were examined for TgAb in the three methods. The nosological sensitivity for ELISA methods was comparable to that of RIA and higher than that of TRC. The ELISA method was accurate, precise, objective, inexpensive and well suited for quantitative routine determination of TgAb.
AB - An enzyme immunoassay (ELISA) has been developed for the detection of human autoantibodies to thyroglobulin (TgAb). The ELISA assay was compared to the previous routine method, the tanned red cell (TRC) method and a radioimmunoassay (RIA) technique for thyroglobulin autoantibodies. Sera from 163 healthy non-hospitalized persons, 31 patients with Hashimoto's thyroiditis, 13 patients with Graves disease, 136 patients with thyroid cancer and 365 sera consecutively received for thyroid autoantibody screening were examined for TgAb in the three methods. The nosological sensitivity for ELISA methods was comparable to that of RIA and higher than that of TRC. The ELISA method was accurate, precise, objective, inexpensive and well suited for quantitative routine determination of TgAb.
KW - Autoantibodies/analysis
KW - Enzyme-Linked Immunosorbent Assay
KW - Graves Disease/immunology
KW - Hemagglutination Tests
KW - Humans
KW - Immunoenzyme Techniques
KW - Radioimmunoassay
KW - Thyroglobulin/immunology
KW - Thyroid Neoplasms/immunology
KW - Thyroiditis, Autoimmune/immunology
U2 - 10.1111/j.1699-0463.1984.tb00103.x
DO - 10.1111/j.1699-0463.1984.tb00103.x
M3 - Journal article
C2 - 6395631
SN - 0108-0202
VL - 92
SP - 377
EP - 382
JO - Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology
JF - Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology
IS - 6
ER -