Endothelial-specific loss of Sphingosine-1-Phosphate Receptor 1 increases vascular permeability and exacerbates Bleomycin-induced pulmonary fibrosis

Rachel S Knipe, Jillian J Spinney, Elizabeth A Abe, Clemens K Probst, Alicia Franklin, Amanda Logue, Francesca Giacona, Matt Drummond, Jason Griffith, Patricia L Brazee, Lida P Hariri, Sydney B Montesi, Katherine E Black, Timothy Hla, Andrew Kuo, Andreane Cartier, Eric Engelbrecht, Christina Christoffersen, Barry S Shea, Andrew M TagerBenjamin D Medoff

4 Citationer (Scopus)

Abstrakt

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive disease which leads to significant morbidity and mortality from respiratory failure. The two drugs currently approved for clinical use slow the rate of decline in lung function but have not been shown to halt disease progression or reverse established fibrosis. Thus, new therapeutic targets are needed. Endothelial injury and the resultant vascular permeability are critical components in the response to tissue injury and are present in patients with IPF. However, it remains unclear how vascular permeability affects lung repair and fibrosis following injury. Lipid mediators such as sphingosine-1-phosphate (S1P) are known to regulate multiple homeostatic processes in the lung including vascular permeability. We demonstrate that endothelial cell-(EC) specific deletion of the S1P receptor 1 (S1PR1) in mice (EC-S1pr1-/-) results in increased lung vascular permeability at baseline. Following a low-dose intratracheal bleomycin challenge, EC-S1pr1-/- mice had increased and persistent vascular permeability compared with wild-type mice, which was strongly correlated with the amount and localization of resulting pulmonary fibrosis. EC-S1pr1-/- mice also had increased immune cell infiltration and activation of the coagulation cascade within the lung. However, increased circulating S1P ligand in ApoM-overexpressing mice was insufficient to protect against bleomycin-induced pulmonary fibrosis. Overall, these data demonstrate that endothelial cell S1PR1 controls vascular permeability in the lung, is associated with changes in immune cell infiltration and extravascular coagulation, and modulates the fibrotic response to lung injury.

OriginalsprogEngelsk
TidsskriftAmerican Journal of Respiratory Cell and Molecular Biology
Vol/bind66
Udgave nummer1
Sider (fra-til)38-52
Antal sider15
ISSN1044-1549
DOI
StatusUdgivet - jan. 2022

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