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Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria

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@article{bfb8cea8121b46fa8383c536d18ed563,
title = "Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria",
abstract = "We hypothesized that the glycocalyx, which is important for endothelial integrity, is lost in severe malaria. C57BL/6 mice were infected with Plasmodium berghei ANKA, resulting in cerebral malaria, or P. chabaudi AS, resulting in uncomplicated malaria. We visualized the glycocalyx with transmission electron microscopy and measured circulating glycosaminoglycans by dot blot and ELISA. The glycocalyx was degraded in brain vasculature in cerebral and to a lesser degree uncomplicated malaria. It was affected on both intact and apoptotic endothelial cells. Circulating glycosaminoglycan levels suggested that glycocalyx disruption preceded cerebral manifestations. The contribution of this loss to pathogenesis should be studied further.",
keywords = "Animals, Disease Models, Animal, Endothelial Cells, Enzyme-Linked Immunosorbent Assay, Female, Glycocalyx, Glycosaminoglycans, Immunoblotting, Malaria, Cerebral, Mice, Mice, Inbred C57BL, Microscopy, Electron, Transmission",
author = "Casper Hempel and Poul Hyttel and Kurtzhals, {J{\o}rgen A L}",
year = "2014",
month = "7",
doi = "10.1038/jcbfm.2014.79",
language = "English",
volume = "34",
pages = "1107--10",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "7",

}

RIS

TY - JOUR

T1 - Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria

AU - Hempel, Casper

AU - Hyttel, Poul

AU - Kurtzhals, Jørgen A L

PY - 2014/7

Y1 - 2014/7

N2 - We hypothesized that the glycocalyx, which is important for endothelial integrity, is lost in severe malaria. C57BL/6 mice were infected with Plasmodium berghei ANKA, resulting in cerebral malaria, or P. chabaudi AS, resulting in uncomplicated malaria. We visualized the glycocalyx with transmission electron microscopy and measured circulating glycosaminoglycans by dot blot and ELISA. The glycocalyx was degraded in brain vasculature in cerebral and to a lesser degree uncomplicated malaria. It was affected on both intact and apoptotic endothelial cells. Circulating glycosaminoglycan levels suggested that glycocalyx disruption preceded cerebral manifestations. The contribution of this loss to pathogenesis should be studied further.

AB - We hypothesized that the glycocalyx, which is important for endothelial integrity, is lost in severe malaria. C57BL/6 mice were infected with Plasmodium berghei ANKA, resulting in cerebral malaria, or P. chabaudi AS, resulting in uncomplicated malaria. We visualized the glycocalyx with transmission electron microscopy and measured circulating glycosaminoglycans by dot blot and ELISA. The glycocalyx was degraded in brain vasculature in cerebral and to a lesser degree uncomplicated malaria. It was affected on both intact and apoptotic endothelial cells. Circulating glycosaminoglycan levels suggested that glycocalyx disruption preceded cerebral manifestations. The contribution of this loss to pathogenesis should be studied further.

KW - Animals

KW - Disease Models, Animal

KW - Endothelial Cells

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Glycocalyx

KW - Glycosaminoglycans

KW - Immunoblotting

KW - Malaria, Cerebral

KW - Mice

KW - Mice, Inbred C57BL

KW - Microscopy, Electron, Transmission

U2 - 10.1038/jcbfm.2014.79

DO - 10.1038/jcbfm.2014.79

M3 - Journal article

VL - 34

SP - 1107

EP - 1110

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 7

ER -

ID: 44829266